{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:26:43Z","timestamp":1758925603863},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1996,12,1]]},"abstract":"<jats:p> Simultaneous whole cell patch-clamp and indo 1 fluorescence measurements were used to characterize ATP-evoked membrane currents and intracellular Ca2+ concentration ([Ca2+]i) changes in isolated Hensen cells of the guinea pig organ of Corti. At negative holding potential, ATP activated a biphasic inward current and a concomitant increase in [Ca2+]i. The initial current activated within &lt; 50 ms, showed a reversal potential near 0 mV and was reversibly inhibited by 30 microM suramin, suggesting this conductance was mediated by ATP-gated nonselective cation channels. The delayed ATP-activated current was mainly carried by Cl- as indicated by its shift in reversal potential when intracellular Cl- was replaced by gluconate. This Cl- conductance appeared to be Ca(2+)-activated secondarily to Ca2+ influx, since it required the presence of extracellular Ca2+ and was suppressed when an intracellular solution containing 10 mM 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid was used. In the absence of extracellular Ca2+, ATP still increased [Ca2+]i concomitant with a monophasic inward cation current, indicating Ca2+ release from intracellular stores. We conclude that Hensen cells have ionotropic and metabotropic P2 purinoceptors. They also have Ca(2+)-activated Cl- channels that can be activated by extracellular ATP, suggesting that purinoceptors in Hensen cells could play a regulatory role in ion and water balance of cochlear fluids. <\/jats:p>","DOI":"10.1152\/ajpcell.1996.271.6.c1817","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T19:29:42Z","timestamp":1514143782000},"page":"C1817-C1827","source":"Crossref","is-referenced-by-count":34,"title":["ATP activates a cation conductance and Ca(2+)-dependent Cl- conductance in Hensen cells of guinea pig cochlea"],"prefix":"10.1152","volume":"271","author":[{"given":"M.","family":"Sugasawa","sequence":"first","affiliation":[{"name":"Laboratoire d'Audiologie Experimentale, Institut National de la Sante et de la Recherche Medicale, Hopital Pellegrin, Bordeaux, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Erostegui","sequence":"additional","affiliation":[{"name":"Laboratoire d'Audiologie Experimentale, Institut National de la Sante et de la Recherche Medicale, Hopital Pellegrin, Bordeaux, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Blanchet","sequence":"additional","affiliation":[{"name":"Laboratoire d'Audiologie Experimentale, Institut National de la Sante et de la Recherche Medicale, Hopital Pellegrin, Bordeaux, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Dulon","sequence":"additional","affiliation":[{"name":"Laboratoire d'Audiologie Experimentale, Institut National de la Sante et de la Recherche Medicale, Hopital Pellegrin, Bordeaux, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1996.271.6.C1817","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:35:51Z","timestamp":1718652951000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1996.271.6.C1817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,12,1]]}},"alternative-id":["10.1152\/ajpcell.1996.271.6.C1817"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1996.271.6.c1817","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,12,1]]}}}