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We investigated the early glomerular changes in male Munich-Wistar-Fr\u00f6mter (MWF) rats, which spontaneously develop albuminuria, and male albuminuria-resistant spontaneously hypertensive rats (SHR). In addition, since female MWF rats are protected from overt proteinuria and progressive renal disease, we compared the phenotypic changes in podocytes during early development of albuminuria in male and female MWF rats. In male MWF rats, glomerular hypertrophy preceded the onset of albuminuria and was greater than in male SHR. Albuminuria developed starting at 6 wk of age and coincided with focal and segmental loss of podoplanin, increased expression of desmin, entrapment of albumin in affected podocytes, and focal and segmental foot process effacement at the ultrastructural level. Other podocyte-associated molecules, such as nephrin and zonula occludens 1, were unaffected. Early glomerular hypertrophy and podocyte damage did not differ between male and female MWF rats. Our data show for the first time that albuminuria in male and female MWF rats is preceded by glomerular hypertrophy and accompanied by focal and segmental loss of podoplanin when FSGS was not yet present.<\/jats:p>","DOI":"10.1152\/ajprenal.00457.2007","type":"journal-article","created":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T01:25:58Z","timestamp":1200533158000},"page":"F758-F767","source":"Crossref","is-referenced-by-count":44,"title":["Glomerular hypertrophy precedes albuminuria and segmental loss of podoplanin in podocytes in Munich-Wistar-Fr\u00f6mter rats"],"prefix":"10.1152","volume":"294","author":[{"given":"D. H. T.","family":"IJpelaar","sequence":"first","affiliation":[]},{"given":"A.","family":"Schulz","sequence":"additional","affiliation":[]},{"given":"K.","family":"Koop","sequence":"additional","affiliation":[]},{"given":"M.","family":"Schlesener","sequence":"additional","affiliation":[]},{"given":"J. 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