{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:45:48Z","timestamp":1772894748128,"version":"3.50.1"},"reference-count":26,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Applied Physiology"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:p>8-Isoprostaglandin F<jats:sub>2\u03b1<\/jats:sub>(8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>) is a bioactive lipid peroxidation product that is a vasoconstrictor at high concentrations. Paradoxically, at lower, and possibly physiological, concentrations, it is a pulmonary vascular muscle's relaxant. Its effects on newborn pulmonary vasculature are unknown. We hypothesized that the pulmonary arterial 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>responses may be developmentally regulated. Therefore, the purpose of this study was to evaluate and compare 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>effects between 1- and 2-wk-old newborn and adult rat isolated intrapulmonary arteries (100 \u03bcm) mounted on a myograph. Force after 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>stimulation was greatest in the adult ( P &lt; 0.01). In newborns, force was significantly increased by the nitric oxide (NO) synthase inhibitor N<jats:sup>G<\/jats:sup>-nitro-l-arginine methyl ester (l-NAME) ( P &lt; 0.01) and was suppressed by blockade of the thromboxane (Tx) A<jats:sub>2<\/jats:sub>receptor. Whereas 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>induced a significant dose-dependent relaxation of adult precontracted vessels in the presence of a TxA<jats:sub>2<\/jats:sub>mimetic (U-46619; 1 \u03bcM), contraction was observed in the 1-wk-old rat. This 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>-induced contraction was abolished by endothelium removal and l-NAME and was attenuated by the cyclooxygenase inhibitor ibuprofen. In the presence of a TxA<jats:sub>2<\/jats:sub>\/prostaglandin H<jats:sub>2<\/jats:sub>receptor blocker, 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>induced NO-mediated relaxation, the magnitude of which was greater in the newborn, compared with the adult ( P &lt; 0.01). When exposed to 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>in vitro, only the newborn lung secreted TxB<jats:sub>2<\/jats:sub>. We conclude that, in contrast to its relaxant effect in the adult, 8-iso-PGF<jats:sub>2\u03b1<\/jats:sub>induces contraction of the pulmonary arteries in the early postnatal period, which is likely to be mediated by endothelium-derived TxA<jats:sub>2<\/jats:sub>. This phenomenon may contribute to the maintenance of a higher pulmonary vascular resistance in the early postnatal period.<\/jats:p>","DOI":"10.1152\/japplphysiol.00420.2003","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:47:13Z","timestamp":1425415633000},"page":"1979-1985","source":"Crossref","is-referenced-by-count":21,"title":["Effect of 8-isoprostaglandin F<sub>2\u03b1<\/sub>on the newborn rat pulmonary arterial muscle and endothelium"],"prefix":"10.1152","volume":"95","author":[{"given":"J.","family":"Belik","sequence":"first","affiliation":[]},{"given":"R. P.","family":"Jankov","sequence":"additional","affiliation":[]},{"given":"J.","family":"Pan","sequence":"additional","affiliation":[]},{"given":"M.","family":"Yi","sequence":"additional","affiliation":[]},{"given":"C. R.","family":"Pace-Asciak","sequence":"additional","affiliation":[]},{"given":"A. K.","family":"Tanswell","sequence":"additional","affiliation":[]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Abman SHand Stenmark KR.Changes in lung eicosanoid content during normal and abnormal transition in perinatal lambs.Am J Physiol Lung Cell Mol Physiol262: L214-L222, 1992.","DOI":"10.1152\/ajplung.1992.262.2.L214"},{"key":"REF2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.88.5.2117"},{"key":"REF3","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.69.2.179"},{"key":"REF4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.5.C1424"},{"key":"REF5","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00820.2002"},{"key":"REF6","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.74.2.682"},{"key":"REF7","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.3.1582"},{"key":"REF8","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.7.6.7682524"},{"key":"REF9","doi-asserted-by":"crossref","unstructured":"Eichert K, Hamacher J, Wunder MA, and Wendel A.Intravasal peroxynitrite generation causes dysfunction in the isolated perfused rat lung via endothelin.J Pharmacol Exp Ther297: 128-132, 2001.","DOI":"10.1016\/S0022-3565(24)29519-7"},{"key":"REF10","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199526003-00016"},{"key":"REF11","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1994.267.5.L585"},{"key":"REF12","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.1950070404"},{"key":"REF13","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.31.2.516"},{"key":"REF14","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200112-124OC"},{"key":"REF15","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200108000-00003"},{"key":"REF16","doi-asserted-by":"publisher","DOI":"10.1006\/pupt.2000.0244"},{"key":"REF17","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.280.6.L1067"},{"key":"REF18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701441"},{"key":"REF19","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701052"},{"key":"REF20","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6523"},{"key":"REF21","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.10058"},{"key":"REF22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1998.274.5.R1406"},{"key":"REF23","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(98)00334-7"},{"key":"REF24","doi-asserted-by":"publisher","DOI":"10.1139\/y90-241"},{"key":"REF25","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-6980(97)00127-5"},{"key":"REF26","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00596.x"}],"container-title":["Journal of Applied Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/japplphysiol.00420.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T17:54:10Z","timestamp":1747763650000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/japplphysiol.00420.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,11]]}},"alternative-id":["10.1152\/japplphysiol.00420.2003"],"URL":"https:\/\/doi.org\/10.1152\/japplphysiol.00420.2003","relation":{},"ISSN":["8750-7587","1522-1601"],"issn-type":[{"value":"8750-7587","type":"print"},{"value":"1522-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11]]}}}