{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:38:29Z","timestamp":1791121109447,"version":"4.1.0"},"reference-count":26,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:p> Insulin secretion is dependent on coordinated pancreatic islet physiology. In the present study, we found a way to overcome the limitations of cellular electrophysiology to optically determine cell membrane potential ( V<jats:sub>m<\/jats:sub>) throughout an islet by using a fast voltage optical dye pair. Using laser scanning confocal microscopy (LSCM), we observed fluorescence (F\u00f6rster) resonance energy transfer (FRET) with the fluorescent donor N-(6-chloro-7-hydroxycoumarin-3-carbonyl)-dimyristoylphosphatidyl-ethanolamine and the acceptor bis-(1,3-diethylthiobarbiturate) trimethine oxonol in the plasma membrane of essentially every cell within an islet. The FRET signal was approximately linear from V<jats:sub>m<\/jats:sub> \u221270 to +50 mV with a 2.5-fold change in amplitude. We evaluated the responses of islet cells to glucose and tetraethylammonium. Essentially, every responding cell in a mouse islet displayed similar time-dependent changes in V<jats:sub>m<\/jats:sub>. When V<jats:sub>m<\/jats:sub> was measured simultaneously with intracellular Ca<jats:sup>2+<\/jats:sup>, all active cells showed tight coupling of V<jats:sub>m<\/jats:sub> to islet cell Ca<jats:sup>2+<\/jats:sup> changes. Our findings indicate that FRET-based, voltage-sensitive dyes used in conjunction with LSCM imaging could be extremely useful in studies of excitation-secretion coupling in intact islets of Langerhans. <\/jats:p>","DOI":"10.1152\/ajpcell.00004.2005","type":"journal-article","created":{"date-parts":[[2005,3,10]],"date-time":"2005-03-10T01:59:19Z","timestamp":1110419959000},"page":"C224-C229","source":"Crossref","is-referenced-by-count":30,"title":["FRET-based voltage probes for confocal imaging: membrane potential oscillations throughout pancreatic islets"],"prefix":"10.1152","volume":"289","author":[{"given":"Andrey","family":"Kuznetsov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vytautas P.","family":"Bindokas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy D.","family":"Marks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis H.","family":"Philipson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Asada N, Shibuya I, Iwanaga T, Niwa K, and Kanno T. 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