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Results demonstrate that zymogen granules predominate as an apically polarized population of acidic organelles that contributes to the initial Ca<jats:sup>2+<\/jats:sup>release. Moreover, we provide evidence that indicates a role for the intracellular messenger NAADP in the release of Ca<jats:sup>2+<\/jats:sup>from acidic organelles following elevation of cAMP. Our data are consistent with the \u201ctrigger\u201d hypothesis where localized release of Ca<jats:sup>2+<\/jats:sup>sensitizes canonical intracellular Ca<jats:sup>2+<\/jats:sup>channels to enhance signals from the endoplasmic reticulum. Release from acidic stores may be important for initiating saliva secretion at low levels of stimulation and a potential therapeutic target to augment secretory activity in hypofunctioning salivary glands.<\/jats:p>","DOI":"10.1152\/ajpcell.00010.2016","type":"journal-article","created":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T03:09:18Z","timestamp":1473304158000},"page":"C697-C709","source":"Crossref","is-referenced-by-count":4,"title":["cAMP-dependent recruitment of acidic organelles for Ca<sup>2+<\/sup>signaling in the salivary gland"],"prefix":"10.1152","volume":"311","author":[{"given":"John F.","family":"Imbery","sequence":"first","affiliation":[{"name":"Department of Neurosciences, University of Toledo Medical Center, Toledo, Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumit","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, University of Toledo Medical Center, Toledo, 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