{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:57:39Z","timestamp":1740139059811,"version":"3.37.3"},"reference-count":47,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["INT-VA\/045"],"award-info":[{"award-number":["INT-VA\/045"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:p> Isolated smooth muscle cells (SMCs) from mouse bronchus were studied using the whole cell patch-clamp technique at \u223c21\u00b0C. Stepping from \u2212100 mV to \u221220 mV evoked inward currents of mean amplitude \u2212275 pA. These inactivated (tau = 1.1 ms) and were abolished when external Na<jats:sup>+<\/jats:sup> was substituted with N-Methyl-d-glucamine. In current-voltage protocols, current peaked at \u221210 mV and reversed between +20 and +30 mV. The V<jats:sub>1\/2<\/jats:sub>s of activation and inactivation were \u221225 and \u221286 mV, respectively. The current was highly sensitive to tetrodotoxin (IC<jats:sub>50<\/jats:sub>\u2009=\u20091.5 nM) and the Na<jats:sub>V<\/jats:sub>1.7 subtype-selective blocker, PF-05089771 (IC<jats:sub>50<\/jats:sub>\u2009=\u20098.6 nM), consistent with Na<jats:sub>V<\/jats:sub>1.7 as the underlying pore-forming \u03b1 subunit. Two Na<jats:sub>V<\/jats:sub>1.7-selective antibodies caused membrane-delineated staining of isolated SMC, as did a nonselective pan-Na<jats:sub>V<\/jats:sub> antibody. RT-PCR, performed on groups of \u223c15 isolated SMCs, revealed transcripts for Na<jats:sub>V<\/jats:sub>1.7 in 7\/8 samples. Veratridine (30 \u00b5M), a nonselective Na<jats:sub>V<\/jats:sub> channel activator, reduced peak current evoked by depolarization but induced a sustained current of 40 pA. Both effects were reversed by tetrodotoxin (100 nM). In tension experiments, veratridine (10 \u00b5M) induced contractions that were entirely blocked by atropine (1 \u00b5M). However, in the presence of atropine, veratridine was able to modulate the pattern of activity induced by a combination of U-46619 (a thromboxane A2 mimetic) and PGE<jats:sub>2<\/jats:sub> (prostaglandin E<jats:sub>2<\/jats:sub>), by eliminating bursts in favor of sustained phasic contractions. These effects were readily reversed to control-like activity by tetrodotoxin (100 nM). In conclusion, mouse bronchial SMCs functionally express Na<jats:sub>V<\/jats:sub>1.7 channels that are capable of modulating contractile activity, at least under experimental conditions. <\/jats:p>","DOI":"10.1152\/ajpcell.00011.2022","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T11:20:03Z","timestamp":1658748003000},"page":"C749-C762","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Functional expression of Na<sub>V<\/sub>1.7 channels in freshly dispersed mouse bronchial smooth muscle cells"],"prefix":"10.1152","volume":"323","author":[{"given":"Ruth M.","family":"Matthews","sequence":"first","affiliation":[{"name":"Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eamonn","family":"Bradley","sequence":"additional","affiliation":[{"name":"Smooth Muscle Research Centre, 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