{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:20:42Z","timestamp":1780453242773,"version":"3.54.1"},"reference-count":37,"publisher":"American Physiological Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p> The pineal gland regulates circadian rhythm through the synthesis and secretion of melatonin. The rise of intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) following nicotinic acetylcholine receptor (nAChR) stimulation due to parasympathetic nerve activity downregulates melatonin production. Important characteristics and roles of Ca<jats:sup>2+<\/jats:sup> mobilization due to nAChR stimulation remain to be clarified. We report here that spontaneous Ca<jats:sup>2+<\/jats:sup> oscillations can be observed in \u223c15% of the pinealocytes in slice preparations from rat pineal glands when this dissociation procedure is done within 6 h from a dark-to-light change. The frequency and half-life of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> rise were 0.86 min<jats:sup>\u22121<\/jats:sup> and 19 s, respectively. Similar spontaneous Ca<jats:sup>2+<\/jats:sup> oscillations were recorded in 17% of rat pinealocytes that were primary cultured for several days. Simultaneous measurement of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and membrane potential revealed that spontaneous Ca<jats:sup>2+<\/jats:sup> oscillations were triggered by periodic membrane depolarizations. Spontaneous Ca<jats:sup>2+<\/jats:sup> oscillations in cultured pinealocytes were abolished by extracellular Ca<jats:sup>2+<\/jats:sup> removal or application of nifedipine, a blocker of voltage-dependent Ca<jats:sup>2+<\/jats:sup> channel (VDCC). In contrast, blockers of intracellular Ca<jats:sup>2+<\/jats:sup>-release channels, 2-aminoethoxydiphenylborate and ryanodine, have no effect. Our results also reveal that, in 23% quiescent pinealocytes, Ca<jats:sup>2+<\/jats:sup> oscillations were observed following the withdrawal of nicotine. Norepinephrine-induced melatonin secretion from whole pineal glands was significantly decreased by the coapplication of acetylcholine (ACh). This inhibitory effect of ACh was attenuated by nifedipine. In conclusion, both spontaneous and evoked Ca<jats:sup>2+<\/jats:sup> oscillations are due to membrane depolarization following activation of VDCCs. This consists of VDCC \u03b11F subunit, and the associated Ca<jats:sup>2+<\/jats:sup> influx can strongly regulate melatonin secretion in pineal glands. <\/jats:p>","DOI":"10.1152\/ajpcell.00014.2014","type":"journal-article","created":{"date-parts":[[2014,4,3]],"date-time":"2014-04-03T01:22:44Z","timestamp":1396488164000},"page":"C1008-C1016","source":"Crossref","is-referenced-by-count":17,"title":["Spontaneous and nicotine-induced Ca<sup>2+<\/sup> oscillations mediated by Ca<sup>2+<\/sup> influx in rat pinealocytes"],"prefix":"10.1152","volume":"306","author":[{"given":"Hiroya","family":"Mizutani","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisao","family":"Yamamura","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan; 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