{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:49:50Z","timestamp":1773211790293,"version":"3.50.1"},"reference-count":41,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2013,7,15]]},"abstract":"<jats:p> The activity of the plasmalemmal Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger (NCX) is highly sensitive to temperature. We took advantage of this fact to explore here the effects of the NCX blocker KB-R7943 (KBR) at 22 and 37\u00b0C on the kinetics of Ca<jats:sup>2+<\/jats:sup> currents ( I<jats:sub>Ca<\/jats:sub>), cytosolic Ca<jats:sup>2+<\/jats:sup> ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>c<\/jats:sub>) transients, and catecholamine release from bovine chromaffin cells (BCCs) stimulated with high K<jats:sup>+<\/jats:sup>, caffeine, or histamine. At 22\u00b0C, the effects of KBR on those parameters were meager or nil. However, at 37\u00b0C whereby the NCX is moving Ca<jats:sup>2+<\/jats:sup> at a rate fivefold higher than at 22\u00b0C, various of the effects of KBR were pronounced, namely: 1) no effects on I<jats:sub>Ca<\/jats:sub>; 2) reduction of the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>c<\/jats:sub> transient amplitude and slowing down of its rate of clearance; 3) blockade of the K<jats:sup>+<\/jats:sup>-elicited quantal release of catecholamine; 4) blockade of burst catecholamine release elicited by K<jats:sup>+<\/jats:sup>; 5) no effect on catecholamine release elicited by short K<jats:sup>+<\/jats:sup> pulses (1\u20132 s) and blockade of the responses produced by longer K<jats:sup>+<\/jats:sup> pulses (3\u20135 s); and 6) potentiation of secretion elicited by histamine or caffeine. Furthermore, the more selective NCX blocker SEA0400 also potentiated the secretory responses to caffeine. The results suggest that at physiological temperature the NCX substantially contributes to shaping the kinetics of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>c<\/jats:sub> transients and the exocytotic responses elicited by Ca<jats:sup>2+<\/jats:sup> entry through Ca<jats:sup>2+<\/jats:sup> channels as well as by Ca<jats:sup>2+<\/jats:sup> release from the endoplasmic reticulum. <\/jats:p>","DOI":"10.1152\/ajpcell.00016.2013","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T01:28:19Z","timestamp":1366248499000},"page":"C160-C172","source":"Crossref","is-referenced-by-count":9,"title":["Plasmalemmal sodium-calcium exchanger shapes the calcium and exocytotic signals of chromaffin cells at physiological temperature"],"prefix":"10.1152","volume":"305","author":[{"given":"Juan-Fernando","family":"Pad\u00edn","sequence":"first","affiliation":[{"name":"Instituto Te\u00f3filo Hernando, Universidad Aut\u00f3noma de Madrid, Madrid, Spain;"},{"name":"Departamento de Farmacolog\u00eda y Terap\u00e9utica, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid, Madrid, Spain;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9-Carlos","family":"Fern\u00e1ndez-Morales","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando, Universidad Aut\u00f3noma de Madrid, Madrid, Spain;"},{"name":"Departamento de Farmacolog\u00eda y Terap\u00e9utica, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid, Madrid, Spain;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rom\u00e1n","family":"Olivares","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencia, Universidad Miguel Hern\u00e1ndez, Alicante, Spain; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Vestring","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando, Universidad Aut\u00f3noma de Madrid, Madrid, Spain;"},{"name":"Medizinische Fakult\u00e4t Carl Gustav Carus, Technische Universit\u00e4t Dresden, Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan-Alberto","family":"Arranz-Tagarro","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando, Universidad Aut\u00f3noma de Madrid, Madrid, Spain;"},{"name":"Departamento de Farmacolog\u00eda y Terap\u00e9utica, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid, Madrid, Spain;"},{"name":"Departamento de Farmacolog\u00eda, Facultad de Farmacia. 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