{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T06:17:34Z","timestamp":1771049854392,"version":"3.50.1"},"reference-count":48,"publisher":"American Physiological Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:p> The aim of this study was to define the effects of polysulfide on intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and the underlying machinery, especially from the hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) and nitric oxide (NO) perspectives, in rat peritoneal mast cells. We found that a polysulfide donor, Na<jats:sub>2<\/jats:sub>S<jats:sub>4<\/jats:sub>, increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, which is both extracellular and intracellular Ca<jats:sup>2+<\/jats:sup> dependent. Intracellular Ca<jats:sup>2+<\/jats:sup> release induced by Na<jats:sub>2<\/jats:sub>S<jats:sub>4<\/jats:sub> was attenuated by the addition of a ryanodine receptor blocker. A slow-releasing H<jats:sub>2<\/jats:sub>S donor, GYY4137, dose dependently increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> that was independent from extracellular Ca<jats:sup>2+<\/jats:sup> influx. The GYY4137-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> release was partially attenuated in the presence of the ryanodine receptor blocker. Both polysulfide and H<jats:sub>2<\/jats:sub>S donors increased the intracellular NO levels in DAF-2-loaded mast cells, which were abolished by an NO scavenger, cPTIO. Inhibition of NO synthase (NOS) significantly abolished the polysulfide- or H<jats:sub>2<\/jats:sub>S-donor-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> elevation in the absence of extracellular Ca<jats:sup>2+<\/jats:sup>. An NO donor, diethylamine (DEA) NONOate, increased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in a concentration-dependent manner, in which both extracellular and intracellular Ca<jats:sup>2+<\/jats:sup> are associated. At higher concentrations, the DEA NONOate-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increases were attenuated in the absence of extracellular Ca<jats:sup>2+<\/jats:sup> and by the addition of the ryanodine receptor blocker. H<jats:sub>2<\/jats:sub>S and NO dose dependently induced polysulfide production. Curiously, polysulfide, H<jats:sub>2<\/jats:sub>S, and NO donors had no effect on mast cell degranulation. Among synthases, cystathionine-\u03b3-lyase, and neuronal NOS seemed to be the major H<jats:sub>2<\/jats:sub>S- and NO-producing synthases, respectively. These results indicate that polysulfide acts as a potential signaling molecule that regulates [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> homeostasis in rat peritoneal mast cells via a cross talk with NO and H<jats:sub>2<\/jats:sub>S. <\/jats:p>","DOI":"10.1152\/ajpcell.00028.2016","type":"journal-article","created":{"date-parts":[[2016,4,7]],"date-time":"2016-04-07T05:28:47Z","timestamp":1460006927000},"page":"C894-C902","source":"Crossref","is-referenced-by-count":19,"title":["Cross talk between polysulfide and nitric oxide in rat peritoneal mast cells"],"prefix":"10.1152","volume":"310","author":[{"given":"Amira","family":"Moustafa","sequence":"first","affiliation":[{"name":"Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan; and"},{"name":"Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Habara","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan; 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