{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T15:06:27Z","timestamp":1749654387158,"version":"3.37.3"},"reference-count":22,"publisher":"American Physiological Society","issue":"6","funder":[{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["PO1 DK41315"],"award-info":[{"award-number":["PO1 DK41315"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20 GM130459"],"award-info":[{"award-number":["P20 GM130459"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2021,6,1]]},"abstract":"<jats:p> Two types of voltage-dependent inward currents were evoked by depolarization in murine antral smooth muscle cells (SMCs) bathed in Ca<jats:sup>2+<\/jats:sup>-containing physiological solution: high-voltage-activated (HVA) and low-voltage-activated (LVA) inward currents. We examined whether the LVA current was due to: 1) T-type Ca<jats:sup>2+<\/jats:sup> channels, 2) Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup>channels, 3) nonselective cation channels (NSCC), or 4) voltage-dependent K<jats:sup>+<\/jats:sup> channels. Replacement of external Ca<jats:sup>2+<\/jats:sup> (2 mM) with equimolar Ba<jats:sup>2+<\/jats:sup> increased the amplitude of the HVA current but blocked the LVA current. Nicardipine blocked the HVA current, and in the presence of nicardipine, T-type Ca<jats:sup>2+<\/jats:sup> blockers failed to block LVA current. A Cl<jats:sup>\u2212<\/jats:sup> channel antagonist had little effect on LVA current. Cation-free external solution completely abolished both HVA and LVA currents. Addition of Ca<jats:sup>2+<\/jats:sup> to the solution restored only HVA currents. Addition of K<jats:sup>+<\/jats:sup> (5 mM) to otherwise cation-free solution induced LVA current that reversed at \u221220 mV. These data suggest that LVA current is not due to T-type Ca<jats:sup>2+<\/jats:sup> channels, Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup> channels, or NSCC. A-type K<jats:sup>+<\/jats:sup> (K<jats:sub>A<\/jats:sub>) currents and delayed rectifying K<jats:sup>+<\/jats:sup> (K<jats:sub>DR<\/jats:sub>) currents can be resolved in antral SMCs dialyzed with a solution containing 140\u2009mM K<jats:sup>+<\/jats:sup>. When cells were exposed to high K<jats:sup>+<\/jats:sup> external solution and dialyzed with Cs<jats:sup>+<\/jats:sup>-rich solution in the presence of nicardipine, LVA current was evoked and reversed at positive potentials. LVA currents were blocked by K<jats:sup>+<\/jats:sup> channel blockers, 4-aminopyridine, and tetraethylammonium. In conclusion, LVA inward currents can be generated by K<jats:sup>+<\/jats:sup> influx via K<jats:sub>A<\/jats:sub> channels in murine antral SMCs when cells were dialyzed with Cs<jats:sup>+<\/jats:sup>-rich solution. <\/jats:p>","DOI":"10.1152\/ajpcell.00031.2021","type":"journal-article","created":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T19:37:22Z","timestamp":1617219442000},"page":"C966-C973","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Low-voltage-activated inward current in murine antral smooth muscle cells is an artifact"],"prefix":"10.1152","volume":"320","author":[{"given":"Ji Yeon","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifeng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenton M.","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Don","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00349.2005"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00074.2009"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2011.058248"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.176206"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00390.2013"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00037.2013"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.156836"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00301.2002"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.025171"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.2170\/jjphysiol.53.165"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1124\/pr.114.009654"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200809986"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200809988"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0130012"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003120"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13431"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1991.66.3.839"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2002"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00849.x"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.107.020115"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021842"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77891-9"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00031.2021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T16:04:37Z","timestamp":1718640277000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00031.2021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,1]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6,1]]}},"alternative-id":["10.1152\/ajpcell.00031.2021"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00031.2021","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"type":"print","value":"0363-6143"},{"type":"electronic","value":"1522-1563"}],"subject":[],"published":{"date-parts":[[2021,6,1]]},"assertion":[{"value":"2021-01-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}