{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T16:02:25Z","timestamp":1788969745841,"version":"build-2803163510"},"reference-count":45,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2012,8,15]]},"abstract":"<jats:p> Acid-sensing ion channels (ASICs) have been reported to play a role in the neuronal dopamine pathway, but the exact role in neurotransmitter release remains elusive. Human neuroblastoma SH-SY5Y is a dopaminergic neuronal cell line, which can release monoamine neurotransmitters. In this study, the expression of ASICs was identified in SH-SY5Y cells to further explore the role of ASICs in vesicular release stimulated by acid. We gathered evidence that ASICs could be detected in SH-SY5Y cells. In whole cell patch-clamp recording, a rapid decrease in extracellular pH evoked inward currents, which were reversibly inhibited by 100 \u03bcM amiloride. The currents were pH dependent, with a pH of half-maximal activation (pH<jats:sub>0.5<\/jats:sub>) of 6.01 \u00b1 0.04. Furthermore, in calcium imaging and FM 1-43 dye labeling, it was shown that extracellular protons increased intracellular calcium levels and vesicular release in SH-SY5Y cells, which was attenuated by PcTx1 and amiloride. Interestingly, N-type calcium channel blockers inhibited the vesicular release induced by acidification. In conclusion, ASICs are functionally expressed in SH-SY5Y cells and involved in vesicular release stimulated by acidification. N-type calcium channels may be involved in the increase in vesicular release induced by acid. Our results provide a preliminary study on ASICs in SH-SY5Y cells and neurotransmitter release, which helps to further investigate the relationship between ASICs and dopaminergic neurons. <\/jats:p>","DOI":"10.1152\/ajpcell.00067.2012","type":"journal-article","created":{"date-parts":[[2012,5,17]],"date-time":"2012-05-17T17:21:43Z","timestamp":1337275303000},"page":"C376-C384","source":"Crossref","is-referenced-by-count":13,"title":["Acid-sensing ion channels contribute to the increase in vesicular release from SH-SY5Y cells stimulated by extracellular protons"],"prefix":"10.1152","volume":"303","author":[{"given":"Qiu-Ju","family":"Xiong","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuang-Li","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, 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