{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T15:55:31Z","timestamp":1761580531167},"reference-count":31,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:p> Primary human mammary epithelial (HME) cells were immortalized by stable, constitutive expression of the catalytic subunit of human telomerase. Purinergic receptors were identified by RT-PCR and quantitative RT-PCR from mRNA isolated from primary and immortalized cells grown to confluence on membrane filters. Several subtypes of P2Y receptor mRNA were identified including P2Y<jats:sub>1<\/jats:sub>, P2Y<jats:sub>2<\/jats:sub>, P2Y<jats:sub>4<\/jats:sub>, and P2Y<jats:sub>6<\/jats:sub> receptors. RT-PCR experiments also revealed expression of A<jats:sub>2b<\/jats:sub> adenosine receptor mRNA in primary and immortalized cells. Confluent monolayers of HME cells exhibited a basal short-circuit current ( I<jats:sub>sc<\/jats:sub>) that was abolished by amiloride and benzamil. When monolayers were cultured in the presence of hydrocortisone, mRNA expression of Na<jats:sup>+<\/jats:sup> channel (ENaC) \u03b1-, \u03b2-, and \u03b3-subunits increased approximately threefold compared with that in cells grown without hydrocortisone. In addition, basal benzamil-sensitive Na<jats:sup>+<\/jats:sup> transport was nearly twofold greater in hydrocortisone-treated monolayers. Stimulation with UTP, UDP, or adenosine 5\u2032- O-(3-thiotriphosphate) (ATP\u03b3S) produced increases in intracellular calcium concentration that were significantly reduced following pretreatment with the calcium-chelating agent BAPTA-AM. Concentration-response relationships indicated that the rank order of potency for these agonists was UTP &gt; UDP &gt; ATP\u03b3S. Basolateral stimulation with UTP produced a rapid but transient increase in I<jats:sub>sc<\/jats:sub> that was significantly reduced if cells were pretreated with BAPTA-AM or benzamil. Moreover, basolateral treatment with either charybdotoxin or clotrimazole significantly inhibited the initial UTP-dependent increase in I<jats:sub>sc<\/jats:sub> and eliminated the sustained current response. These results indicate that human mammary epithelial cells express multiple P2 receptor subtypes and that Ca<jats:sup>2+<\/jats:sup> mobilization evoked by P2Y receptor agonists stimulates Na<jats:sup>+<\/jats:sup> absorption by increasing the activity of Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup> channels located in the basolateral membrane. <\/jats:p>","DOI":"10.1152\/ajpcell.00068.2007","type":"journal-article","created":{"date-parts":[[2007,8,23]],"date-time":"2007-08-23T00:58:37Z","timestamp":1187830717000},"page":"C1472-C1480","source":"Crossref","is-referenced-by-count":22,"title":["P2Y receptor regulation of sodium transport in human mammary epithelial cells"],"prefix":"10.1152","volume":"293","author":[{"given":"So Yeong","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa L.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Maniak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo Hwa","family":"Jang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan Dong","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott M.","family":"O'Grady","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Bernard K, Bogliolo S, Soriani O, Ehrenfeld J. 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