{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:47:38Z","timestamp":1790855258935,"version":"4.1.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p>\n                    Alternative splicing of ryanodine receptor subtype 3 (RYR3) may generate a short isoform (RYR3S) without channel function and a functional full-length isoform (RYR3L). The RYR3S isoform has been shown to negatively regulate the native RYR2 subtype in smooth muscle cells as well as the RYR3L isoform when both isoforms were coexpressed in HEK-293 cells. Mouse myometrium expresses only the RYR3 subtype, but the role of RYR3 isoforms obtained by alternative splicing and their activation by cADP-ribose during pregnancy have never been investigated. Here, we show that both RYR3S and RYR3L isoforms are differentially expressed in nonpregnant and pregnant mouse myometrium. The use of antisense oligonucleotides directed against each isoform indicated that only RYR3L was activated by caffeine and cADP-ribose in nonpregnant myometrium. These RYR3L-mediated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    releases were negatively regulated by RYR3S expression. At the end of pregnancy, the relative expression of RYR3L versus RYR3S and its ability to respond to cADP-ribose were increased. Therefore, our results suggest that physiological regulation of RYR3 alternative splicing may play an essential role at the end of pregnancy.\n                  <\/jats:p>","DOI":"10.1152\/ajpcell.00069.2007","type":"journal-article","created":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T00:43:23Z","timestamp":1182991403000},"page":"C848-C854","source":"Crossref","is-referenced-by-count":32,"title":["Role of RYR3 splice variants in calcium signaling in mouse nonpregnant and pregnant myometrium"],"prefix":"10.1152","volume":"293","author":[{"given":"Fabrice","family":"Dabertrand","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Fritz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Mironneau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Macrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Morel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Aoyama M\n                      , Yamada A, Wang J, Ohya S, Furuzono S, Goto T, Hotta S, Ito Y, Matsubara T, Shimokata K, Chen SR, Imaizumi Y, Nakayama S.\n                      Requirement of ryanodine receptors for pacemaker Ca\n                      2+\n                      activity in ICC and HEK293 cells.\n                      J Cell Sci\n                      117\n                      : 2813\u20132825, 2004.","DOI":"10.1242\/jcs.01136"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Arnaudeau S\n                      , Lepretre N, Mironneau J.\n                      Oxytocin mobilizes calcium from a unique heparin-sensitive and thapsigargin-sensitive store in single myometrial cells from pregnant rats.\n                      Pfl\u00fcgers Arch\n                      428\n                      : 51\u201359, 1994.","DOI":"10.1007\/BF00374751"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Barata H\n                      , Thompson M, Zielinska W, Han YS, Mantilla CB, Prakash YS, Feitoza S, Sieck G, Chini EN.\n                      The role of cyclic-ADP-ribose-signaling pathway in oxytocin-induced Ca\n                      2+\n                      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2000.","DOI":"10.1074\/jbc.275.13.9596"},{"key":"R9","doi-asserted-by":"crossref","unstructured":"Dabertrand F\n                      , Morel JL, Sorrentino V, Mironneau J, Mironneau C, Macrez N.\n                      Modulation of calcium signalling by dominant negative splice variant of ryanodine receptor subtype 3 in native smooth muscle cells.\n                      Cell Calcium\n                      40\n                      : 11\u201321, 2006.","DOI":"10.1016\/j.ceca.2006.03.008"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Dogan S\n                      , Deshpande DA, Kannnan MS, Walseth TF.\n                      Changes in CD38 expression and ADP-ribosyl cyclase activity in rat myometrium during pregnancy: influence of sex hormones.\n                      Biol Reprod\n                      71\n                      : 97\u2013103, 2004.","DOI":"10.1095\/biolreprod.103.026245"},{"key":"R11","doi-asserted-by":"crossref","unstructured":"Dogan S\n                   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channels.\n                      Physiol Rev\n                      82\n                      : 893\u2013922, 2002.","DOI":"10.1152\/physrev.00013.2002"},{"key":"R14","doi-asserted-by":"crossref","unstructured":"Fritz N\n                      , Macrez N, Mironneau J, Jeyakumar LH, Fleischer S, Morel JL.\n                      Ryanodine receptor subtype 2 encodes Ca\n                      2+\n                      oscillations activated by acetylcholine via the M2 muscarinic receptor\/cADP-ribose signalling pathway in duodenum myocytes.\n                      J Cell Sci\n                      118\n                      : 2261\u20132270, 2005.","DOI":"10.1242\/jcs.02344"},{"key":"R15","doi-asserted-by":"crossref","unstructured":"Guse AH\n                      .\n                      Regulation of calcium signaling by the second messenger cyclic adenosine diphosphoribose (cADPR).\n                      Curr Mol Med\n                      4\n                      : 239\u2013248, 2004.","DOI":"10.2174\/1566524043360771"},{"key":"R16","doi-asserted-by":"crossref","unstructured":"Holda JR\n                      , Oberti C, Perez-Reyes E, Blatter LA.\n                      Characterization of an oxytocin-induced rise in [Ca\n                      2+\n                      ]\n                      i\n                      in single human myometrium smooth muscle cells.\n                      Cell Calcium\n                      20\n                      : 43\u201351, 1996.","DOI":"10.1016\/S0143-4160(96)90049-4"},{"key":"R17","doi-asserted-by":"crossref","unstructured":"Jiang D\n                      , Xiao B, Li X, Chen SR.\n                      Smooth muscle tissues express a major dominant negative splice variant of the type 3 Ca\n                      2+\n                      release channel (ryanodine receptor).\n                      J Biol Chem\n                      278\n                      : 4763\u20134769, 2003.","DOI":"10.1074\/jbc.M210410200"},{"key":"R18","doi-asserted-by":"crossref","unstructured":"Kunerth S\n                      , Langhorst MF, Schwarzmann N, Gu X, Huang L, Yang Z, Zhang L, Mills SJ, Zhang LH, Potter BV, Guse AH.\n                      Amplification and propagation of pacemaker Ca\n                      2+\n                      signals by cyclic ADP-ribose and the type 3 ryanodine receptor in T cells.\n                      J Cell Sci\n                      117\n                      : 2141\u20132149, 2004.","DOI":"10.1242\/jcs.01063"},{"key":"R19","doi-asserted-by":"crossref","unstructured":"Lartey J\n                      , Smith M, Pawade J, Strachan B, Mellor H, L\u00f3pez-Bernal A.\n                      Up-regulation of myometrial RHO effector proteins (PKN1 and DIAPH1) and CPI-17 (PPP1R14A) phosphorylation in human pregnancy is associated with increased GTP-RHOA in spontaneous preterm labor.\n                      Biol Reprod\n                      76\n                      : 971\u2013982, 2007.","DOI":"10.1095\/biolreprod.106.058982"},{"key":"R20","doi-asserted-by":"crossref","unstructured":"Lynn S\n                      , Morgan JM, Gillespie JI, Greenwell JR.\n                      A novel ryanodine sensitive calcium release mechanism in cultured human myometrial smooth-muscle cells.\n                      FEBS Lett\n                      330\n                      : 227\u2013230, 1993.","DOI":"10.1016\/0014-5793(93)80279-4"},{"key":"R21","doi-asserted-by":"crossref","unstructured":"Macrez N\n                      , Mironneau J.\n                      Local Ca\n                      2+\n                      signals in cellular signalling.\n                      Curr Mol Med\n                      4\n                      : 263\u2013275, 2004.","DOI":"10.2174\/1566524043360762"},{"key":"R22","doi-asserted-by":"crossref","unstructured":"Marc S\n                      , Leiber D, Harbon S.\n                      Carbachol and oxytocin stimulate the generation of inositol phosphates in the guinea pig myometrium.\n                      FEBS Lett\n                      201\n                      : 9\u201314, 1986.","DOI":"10.1016\/0014-5793(86)80561-0"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Martin C\n                      , Chapman KE, Thornton S, Ashley RH.\n                      Changes in the expression of myometrial ryanodine receptor mRNAs during human pregnancy.\n                      Biochim Biophys Acta\n                      1451\n                      : 343\u2013352, 1999b.","DOI":"10.1016\/S0167-4889(99)00104-4"},{"key":"R24","doi-asserted-by":"crossref","unstructured":"Martin C\n                      , Hyvelin JM, Chapman KE, Marthan R, Ashley RH, Savineau JP.\n                      Pregnant rat myometrial cells show heterogeneous ryanodine- and caffeine-sensitive calcium stores.\n                      Am J Physiol Cell Physiol\n                      277\n                      : C243\u2013C252, 1999.","DOI":"10.1152\/ajpcell.1999.277.2.C243"},{"key":"R25","doi-asserted-by":"crossref","unstructured":"Marziali G\n                      , Rossi D, Giannini G, Charlesworth A, Sorrentino V.\n                      cDNA cloning reveals a tissue specific expression of alternatively spliced transcripts of the ryanodine receptor type 3 (RyR3) calcium release channel.\n                      FEBS Lett\n                      394\n                      : 76\u201382, 1996.","DOI":"10.1016\/0014-5793(96)00944-1"},{"key":"R26","doi-asserted-by":"crossref","unstructured":"Matthew A\n                      , Kupittayanant S, Burdyga T, Wray S.\n                      Characterization of contractile activity and intracellular Ca\n                      2+\n                      signalling in mouse myometrium.\n                      J Soc Gynecol Investig\n                      11\n                      : 207\u2013212, 2004.","DOI":"10.1016\/j.jsgi.2003.10.009"},{"key":"R27","doi-asserted-by":"crossref","unstructured":"Mironneau J\n                      , Coussin F, Jeyakumar LH, Fleischer S, Mironneau C, Macrez N.\n                      Contribution of ryanodine receptor subtype 3 to Ca\n                      2+\n                      responses in Ca\n                      2+\n                      -overloaded cultured rat portal vein myocytes.\n                      J Biol Chem\n                      276\n                      : 11257\u201311264, 2001.","DOI":"10.1074\/jbc.M005994200"},{"key":"R28","doi-asserted-by":"crossref","unstructured":"Mironneau J\n                      , Macrez N, Morel JL, Sorrentino V, Mironneau C.\n                      Identification and function of ryanodine receptor subtype 3 in non-pregnant mouse myometrial cells.\n                      J Physiol\n                      538\n                      : 707\u2013716, 2002.","DOI":"10.1113\/jphysiol.2001.013046"},{"key":"R29","doi-asserted-by":"crossref","unstructured":"Miyatake R\n                      , Furukawa A, Matsushita M, Iwahashi K, Nakamura K, Ichikawa Y, Suwaki H.\n                      Tissue-specific alternative splicing of mouse brain type ryanodine receptor\/calcium release channel mRNA.\n                      FEBS Lett\n                      395\n                      : 123\u2013126, 1996.","DOI":"10.1016\/0014-5793(96)01022-8"},{"key":"R30","doi-asserted-by":"crossref","unstructured":"Morel JL\n                      , Fritz N, Lavie JL, Mironneau J.\n                      Crucial role of type 2 inositol 1,4,5-trisphosphate receptors for acetylcholine-induced Ca\n                      2+\n                      oscillations in vascular myocytes.\n                      Arterioscler Thromb Vasc Biol\n                      23\n                      : 1567\u20131575, 2003.","DOI":"10.1161\/01.ATV.0000089013.82552.5D"},{"key":"R31","doi-asserted-by":"crossref","unstructured":"Morel JL\n                      , Rakotoarisoa L, Jeyakumar LH, Fleischer S, Mironneau C, Mironneau J.\n                      Decreased expression of ryanodine receptors alters calcium-induced calcium release mechanism in mdx duodenal myocytes.\n                      J Biol Chem\n                      279\n                      : 21287\u201321293, 2004.","DOI":"10.1074\/jbc.M311124200"},{"key":"R32","doi-asserted-by":"crossref","unstructured":"Morgan JM\n                      , De Smedt H, Gillespie JI.\n                      Identification of three isoforms of the InsP3 receptor in human myometrial smooth muscle.\n                      Pfl\u00fcgers Arch\n                      431\n                      : 697\u2013705, 1996.","DOI":"10.1007\/BF02253832"},{"key":"R33","doi-asserted-by":"crossref","unstructured":"Ohkubo T\n                      , Inoue Y, Kawarabayashi T, Kitamura K.\n                      Identification and electrophysiological characteristics of isoforms of T-type calcium channel Ca\n                      v\n                      3.2 expressed in pregnant human uterus.\n                      Cell Physiol Biochem\n                      16\n                      : 245\u2013254, 2005.","DOI":"10.1159\/000089850"},{"key":"R34","doi-asserted-by":"crossref","unstructured":"Rossi D\n                      , Simeoni I, Micheli M, Bootman M, Lipp P, Allen PD, Sorrentino V.\n                      RyR1 and RyR3 isoforms provide distinct intracellular Ca\n                      2+\n                      signals in HEK 293 cells.\n                      J Cell Sci\n                      115\n                      : 2497\u20132504, 2002.","DOI":"10.1242\/jcs.115.12.2497"},{"key":"R35","unstructured":"Sanborn BM\n                      , Qian A, Ku CY, Wen Y, Anwer K, Monga M, Singh SP.\n                      Mechanisms regulating oxytocin receptor coupling to phospholipase C in rat and human myometrium.\n                      Adv Exp Med Biol\n                      395\n                      : 469\u2013479, 1995."},{"key":"R36","doi-asserted-by":"crossref","unstructured":"Shmigol AV\n                      , Eisner DA, Wray S.\n                      Simultaneous measurements of changes in sarcoplasmic reticulum and cytosolic.\n                      J Physiol\n                      531\n                      : 707\u2013713, 2001.","DOI":"10.1111\/j.1469-7793.2001.0707h.x"},{"key":"R37","doi-asserted-by":"crossref","unstructured":"Shmygol A\n                      , Wray S.\n                      Modulation of agonist-induced Ca\n                      2+\n                      release by SR Ca\n                      2+\n                      load: direct SR and cytosolic Ca\n                      2+\n                      measurements in rat uterine myocytes.\n                      Cell Calcium\n                      37\n                      : 215\u2013223, 2005.","DOI":"10.1016\/j.ceca.2004.10.002"},{"key":"R38","doi-asserted-by":"crossref","unstructured":"Stuyvers BD\n                      , Dun W, Matkovich S, Sorrentino V, Boyden PA, ter Keurs HE.\n                      Ca\n                      2+\n                      sparks and waves in canine Purkinje cells: a triple layered system of Ca\n                      2+\n                      activation.\n                      Circ Res\n                      97\n                      : 35\u201343, 2005.","DOI":"10.1161\/01.RES.0000173375.26489.fe"},{"key":"R39","doi-asserted-by":"crossref","unstructured":"Taggart MJ\n                      , Wray S.\n                      Contribution of sarcoplasmic reticular calcium to smooth muscle contractile activation: gestational dependence in isolated rat uterus.\n                      J Physiol\n                      511\n                      : 133\u2013144, 1998.","DOI":"10.1111\/j.1469-7793.1998.133bi.x"},{"key":"R40","doi-asserted-by":"crossref","unstructured":"Thompson M\n                      , Barata da Silva H, Zielinska W, White TA, Bailey JP, Lund FE, Sieck GC, Chini EN.\n                      Role of CD38 in myometrial Ca\n                      2+\n                      transients: modulation by progesterone.\n                      Am J Physiol Endocrinol Metab\n                      287\n                      : E1142\u2013E1148, 2004.","DOI":"10.1152\/ajpendo.00122.2004"},{"key":"R41","doi-asserted-by":"crossref","unstructured":"Wray S\n                      , Jones K, Kupittayanant S, Li Y, Matthew A, Monir-Bishty E, Noble K, Pierce SJ, Quenby S, Shmygol AV.\n                      Calcium signaling and uterine contractility.\n                      J Soc Gynecol Investig\n                      10\n                      : 252\u2013264, 2003.","DOI":"10.1016\/S1071-55760300089-3"},{"key":"R42","doi-asserted-by":"crossref","unstructured":"Zhu N\n                      , Eghbali M, Helguera G, Song M, Stefani E, Toro L.\n                      Alternative splicing of Slo channel gene programmed by estrogen, progesterone and pregnancy.\n                      FEBS Lett\n                      579\n                      : 4856\u20134860, 2005.","DOI":"10.1016\/j.febslet.2005.07.069"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00069.2007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T13:53:12Z","timestamp":1718632392000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00069.2007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1152\/ajpcell.00069.2007"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00069.2007","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1094925.549877","asserted-by":"object"}]},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}