{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T08:40:53Z","timestamp":1769244053249,"version":"3.49.0"},"reference-count":50,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/100005384","name":"American Physiological Society (APS)","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005384","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation (NSF)","doi-asserted-by":"publisher","award":["IOS 1354181"],"award-info":[{"award-number":["IOS 1354181"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000879","name":"Alfred P. Sloan Foundation (Sloan Foundation)","doi-asserted-by":"publisher","award":["BR2013-103"],"award-info":[{"award-number":["BR2013-103"]}],"id":[{"id":"10.13039\/100000879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:p> Blood acid-base regulation by specialized epithelia, such as gills and kidney, requires the ability to sense blood acid-base status. Here, we developed primary cultures of ray ( Urolophus halleri) gill cells to study mechanisms for acid-base sensing without the interference of whole animal hormonal regulation. Ray gills have abundant base-secreting cells, identified by their noticeable expression of vacuolar-type H<jats:sup>+<\/jats:sup>-ATPase (VHA), and also express the evolutionarily conserved acid-base sensor soluble adenylyl cyclase (sAC). Exposure of cultured cells to extracellular alkalosis (pH 8.0, 40 mM HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>) triggered VHA translocation to the cell membrane, similar to previous reports in live animals experiencing blood alkalosis. VHA translocation was dependent on sAC, as it was blocked by the sAC-specific inhibitor KH7. Ray gill base-secreting cells also express transmembrane adenylyl cyclases (tmACs); however, tmAC inhibition by 2\u2032,5\u2032-dideoxyadenosine did not prevent alkalosis-dependent VHA translocation, and tmAC activation by forskolin reduced the abundance of VHA at the cell membrane. This study demonstrates that sAC is a necessary and sufficient sensor of extracellular alkalosis in ray gill base-secreting cells. In addition, this study indicates that different sources of cAMP differentially modulate cell biology. <\/jats:p>","DOI":"10.1152\/ajpcell.00089.2016","type":"journal-article","created":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T14:43:42Z","timestamp":1466693022000},"page":"C340-C349","source":"Crossref","is-referenced-by-count":23,"title":["Soluble adenylyl cyclase is an acid-base sensor in epithelial base-secreting cells"],"prefix":"10.1152","volume":"311","author":[{"given":"Jinae N.","family":"Roa","sequence":"first","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Tresguerres","sequence":"additional","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1413483112"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.060003890.x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.113.208496"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261253998"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V92155"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/331622a0"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.1.79"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000172"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1007\/s00360-012-0685-4"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5479.625"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1042\/bj20031061"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.44.27787"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00050.2003"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1979.236.1.F1"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00645.2009"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00059.2011"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-73336-9_8"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.06.007"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/physiologyonline.1988.3.1.28"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1007\/BF00592303"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00317.2013"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00551.2012"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/nature01905"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00604.2005"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309543200"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00406.2007"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00584.2009"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00178.2002"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200810044"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2014.04.003"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.071516798"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1997.16"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.job.2014.11.002"},{"key":"B34","first-page":"201","volume":"7","author":"Seamon KB","year":"1981","journal-title":"J Cyclic Nucleotide Res"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1931.98.2.296"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22926"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009050477"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0865-6"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01382"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2013.0625"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00761.2009"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2011.95"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02059"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911790107"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00814.2006"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90460-3"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00045.2003"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2007.06.008"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01678"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.026450"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00089.2016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T16:16:20Z","timestamp":1718640980000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00089.2016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,1]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,8,1]]}},"alternative-id":["10.1152\/ajpcell.00089.2016"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00089.2016","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,1]]}}}