{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:02:44Z","timestamp":1791295364332,"version":"4.1.0"},"reference-count":48,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:p> COOH-terminal cytoplasmic tails of chloride\/bicarbonate anion exchangers (AE) bind cytosolic carbonic anhydrase II (CAII) to form a bicarbonate transport metabolon, a membrane protein complex that accelerates transmembrane bicarbonate flux. To determine whether interaction with CAII affects the downregulated in adenoma (DRA) chloride\/bicarbonate exchanger, anion exchange activity of DRA-transfected HEK-293 cells was monitored by following changes in intracellular pH associated with bicarbonate transport. DRA-mediated bicarbonate transport activity of 18 \u00b1 1 mM H<jats:sup>+<\/jats:sup> equivalents\/min was inhibited 53 \u00b1 2% by 100 mM of the CAII inhibitor, acetazolamide, but was unaffected by the membrane-impermeant carbonic anhydrase inhibitor, 1-[5-sulfamoyl-1,3,4-thiadiazol-2-yl-(aminosulfonyl-4-phenyl)]-2,6-dimethyl-4-phenyl-pyridinium perchlorate. Compared with AE1, the COOH-terminal tail of DRA interacted weakly with CAII. Overexpression of a functionally inactive CAII mutant, V143Y, reduced AE1 transport activity by 61 \u00b1 4% without effect on DRA transport activity (105 \u00b1 7% transport activity relative to DRA alone). We conclude that cytosolic CAII is required for full DRA-mediated bicarbonate transport. However, DRA differs from other bicarbonate transport proteins because its transport activity is not stimulated by direct interaction with CAII. <\/jats:p>","DOI":"10.1152\/ajpcell.00115.2002","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:11:14Z","timestamp":1368490274000},"page":"C1522-C1529","source":"Crossref","is-referenced-by-count":84,"title":["The functional and physical relationship between the  DRA bicarbonate transporter and carbonic anhydrase II"],"prefix":"10.1152","volume":"283","author":[{"given":"Deborah","family":"Sterling","sequence":"first","affiliation":[{"name":"Canadian Institutes of Health Research Membrane Protein Research Group, Departments of Physiology and Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan J. 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