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With the use of a novel microfluidic device developed in our laboratory, dynamic hypotonic loading (\u2212200 mosM) was applied up to 0.1 Hz and chondrocyte calcium signaling, cytoskeleton organization, and gene expression responses were examined. Chondrocytes exhibited decreasing volume and calcium responses with increasing loading frequency. Phalloidin staining showed osmotic loading-induced changes to the actin cytoskeleton in chondrocytes. Real-time PCR analysis revealed a stimulatory effect of dynamic osmotic loading compared with static osmotic loading. These studies illustrate the utility of the microfluidic device in cell signaling investigations, and their potential role in helping to elucidate mechanisms that mediate chondrocyte mechanotransduction to dynamic stimuli.<\/jats:p>","DOI":"10.1152\/ajpcell.00127.2005","type":"journal-article","created":{"date-parts":[[2006,8,24]],"date-time":"2006-08-24T00:33:37Z","timestamp":1156379617000},"page":"C718-C725","source":"Crossref","is-referenced-by-count":103,"title":["Chondrocyte intracellular calcium, cytoskeletal organization, and gene expression responses to dynamic osmotic loading"],"prefix":"10.1152","volume":"291","author":[{"given":"Pen-hsiu Grace","family":"Chao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan C.","family":"West","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clark 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