{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T23:16:39Z","timestamp":1782947799779,"version":"3.54.5"},"reference-count":53,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK112085"],"award-info":[{"award-number":["DK112085"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK104791"],"award-info":[{"award-number":["DK104791"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2020,2,1]]},"abstract":"<jats:p> Iron deficiency anemia is a common complication of ulcerative colitis (UC) that can profoundly impact quality of life. Most iron absorption occurs in the duodenum via divalent metal transporter 1 (DMT1)-mediated uptake and ferroportin-1 (FPN1)-mediated export across the apical and basolateral membranes, respectively. However, the colon also contains iron transporters and can participate in iron absorption. Studies have shown increased duodenal DMT1 and FPN1 in patients with UC, but there is conflicting evidence about whether expression is altered in UC colon. We hypothesized that expression of colonic DMT1 and FPN1 will also increase to compensate for iron deficiency. Quantitative RT-PCR and Western blot analyses were performed on duodenal and colonic segmental (right colon, transverse colon, left colon, and rectum) biopsies obtained during colonoscopy. DMT1 mRNA and protein abundances in colonic segments were approximately equal to those in the duodenum, whereas colonic FPN1 mRNA and protein abundances of colonic segments were about one-quarter of those of the duodenum. DMT1 specific mRNA and protein abundances were increased twofold, whereas FPN1 mRNA and protein expressions were increased fivefold in UC distal colon. Immunofluorescence studies revealed enhanced expression of apical membrane- and basolateral membrane-localized DMT1 and FPN1 in UC human colon, respectively. Increased DMT1 expression was associated with enhanced 2-(3-carbamimidoylsulfanylmethyl-benzyl)-isothiourea (CISMBI, DMT1 specific inhibitor)-sensitive <jats:sup>59<\/jats:sup>Fe uptake in UC human colon. We conclude from these results that patients with active UC have increased expression of colonic iron transporters and increased iron absorption, which may be targeted in the treatment of UC-related anemia. <\/jats:p>","DOI":"10.1152\/ajpcell.00128.2019","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T21:07:28Z","timestamp":1573679248000},"page":"C263-C271","source":"Crossref","is-referenced-by-count":23,"title":["Increased DMT1 and FPN1 expression with enhanced iron absorption in ulcerative colitis human colon"],"prefix":"10.1152","volume":"318","author":[{"given":"Emily A.","family":"Minor","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology, West Virginia University School of Medicine, Morgantown, West Virginia"},{"name":"Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin T.","family":"Kupec","sequence":"additional","affiliation":[{"name":"Department of Medicine, Digestive Diseases Section, West Virginia University School of Medicine, Morgantown, West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Nickerson","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, West Virginia University School of Medicine, Morgantown, West Virginia"},{"name":"Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karthikeyan","family":"Narayanan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8999-8721","authenticated-orcid":false,"given":"Vazhaikkurichi M.","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, West Virginia University School of Medicine, Morgantown, West Virginia"},{"name":"Department of Medicine, Digestive Diseases Section, West Virginia University School of Medicine, Morgantown, West Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","first-page":"164","volume":"19","author":"Abbaspour N","year":"2014","journal-title":"J Res Med Sci"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1590\/1516-3180.2014.1323568"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.5152\/tjg.2016.16011"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.4638"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1139\/Y07-007"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1080\/003655202320378176"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/s12664-017-0791-z"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.333.7563.340"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2014.01.028"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1006\/bcmd.2002.0564"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e07-01-0060"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2005.01.003"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2008.1.151"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-03-0672"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.01.014"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1177\/1756283X09102329"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i13.3542"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/41343"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i33.6137"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192423399"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000947"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.012809.104801"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.4292\/wjgpt.v1.i2.43"},{"key":"B24","first-page":"124","volume":"20","author":"Kwapisz J","year":"2009","journal-title":"eJIFCC"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2015-309940"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3390\/nu9060617"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0098-2997(00)00006-6"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01671.x"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114514004334"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.crohns.2014.04.008"},{"key":"B31","first-page":"95","volume":"27","author":"Monstad I","year":"2014","journal-title":"Ann Gastroenterol"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1177\/1087057114521663"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.4617"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215686"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(63)80031-1"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1007\/s11938-018-0174-2"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.014399"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2011.232"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2014.00058"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3814"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21796"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2154361"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2015.03.011"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1002\/ccr3.1529"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114513003334"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1080\/00365520510011489"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117383"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0b013e328326cacb"},{"key":"B49","first-page":"51","volume":"37","author":"Wallace DF","year":"2016","journal-title":"Clin Biochem Rev"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00621.2008"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-06-815894"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.03.004"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1038\/srep16344"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00128.2019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T16:18:52Z","timestamp":1718641132000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00128.2019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,1]]},"references-count":53,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2,1]]}},"alternative-id":["10.1152\/ajpcell.00128.2019"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00128.2019","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,1]]}}}