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Cevimeline, a muscarinic receptor agonist upregulated endothelial NO synthase in parotid tissue. NO and H<jats:sub>2<\/jats:sub>S donors increased the intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>). This was not affected by inhibitors of phospholipase C and inositol 1,4,5-trisphosphate receptors, but was decreased by blockers of ryanodine receptors (RyRs), soluble guanylyl cyclase, and protein kinase G. The H<jats:sub>2<\/jats:sub>S donor evoked NO production, which was decreased by blockade of NO synthases or phosphoinositide 3-kinase or by hypotaurine, an H<jats:sub>2<\/jats:sub>S scavenger. The H<jats:sub>2<\/jats:sub>S donor-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>increase was diminished by a NO scavenger or the NO synthases blocker. These results suggest that NO and H<jats:sub>2<\/jats:sub>S play important roles in regulating [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>via soluble guanylyl cyclase-cGMP-protein kinase G-RyRs, but not via inositol 1,4,5-trisphosphate receptors. The effect of H<jats:sub>2<\/jats:sub>S may be partially through NO produced via phosphoinositide 3-kinase-Akt-endothelial NO synthase. It was concluded that both gases regulate [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in a synergistic way, mainly via RyRs in rat parotid acinar cells.<\/jats:p>","DOI":"10.1152\/ajpcell.00147.2015","type":"journal-article","created":{"date-parts":[[2015,7,30]],"date-time":"2015-07-30T03:24:56Z","timestamp":1438226696000},"page":"C480-C490","source":"Crossref","is-referenced-by-count":7,"title":["Hydrogen sulfide: a novel gaseous signaling molecule and intracellular Ca<sup>2+<\/sup>regulator in rat parotid acinar cells"],"prefix":"10.1152","volume":"309","author":[{"given":"Amira","family":"Moustafa","sequence":"first","affiliation":[{"name":"Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan; and"},{"name":"Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 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