{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:38:46Z","timestamp":1787564326480,"version":"build-2736575974"},"reference-count":51,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:p>\n                    The human gastric glandular epithelium produces a gastric lipase enzyme (HGL) that plays an important role in digestion of dietary triglycerides. To assess the involvement of extracellular matrix components and transforming growth factor-\u03b21 (TGF-\u03b21) in the regulation of this enzymic function, normal gastric epithelial cells were cultured on collagen type I, Matrigel, and laminins (LN)-1 and -2 with or without TGF-\u03b21. Epithelial morphology and HGL expression were evaluated using microscopy techniques, enzymic assays, Western blot, Northern hybridization, and RT-PCR. A correlation was observed between the cell polarity status and the level of HGL expression. TGF-\u03b21 alone or individual matrix components stimulated cell spreading and caused a downfall of HGL activity and mRNA. By contrast, Matrigel preserved the morphological features of differentiated epithelial cells and maintained HGL expression. The combination of LNs with TGF-\u03b21 (two constituents of Matrigel) exerted similar beneficial effects on epithelial cell polarity and evoked a 10-fold increase of HGL levels that was blunted by a neutralizing antibody against the \u03b1\n                    <jats:sub>2<\/jats:sub>\n                    -integrin subunit and by mitogen-activated protein kinase (MAPK) inhibitors PD-98059 (p42\/p44) or SB-203580 (p38). This investigation demonstrates for the first time that a powerful synergism between a growth factor and basement membrane LNs positively influences cell polarity and functionality of the human gastric glandular epithelium through an activation of the \u03b1\n                    <jats:sub>2<\/jats:sub>\n                    \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    -integrin and effectors of two MAPK pathways.\n                  <\/jats:p>","DOI":"10.1152\/ajpcell.00150.2001","type":"journal-article","created":{"date-parts":[[2013,5,13]],"date-time":"2013-05-13T20:07:47Z","timestamp":1368475667000},"page":"C873-C884","source":"Crossref","is-referenced-by-count":10,"title":["Laminins and TGF-\u03b2 maintain cell polarity and  functionality of human gastric glandular epithelium"],"prefix":"10.1152","volume":"282","author":[{"given":"Jean-Ren\u00e9","family":"Basque","sequence":"first","affiliation":[{"name":"Canadian Institute of Health Research Group on the Functional Development and Physiopathology of the Digestive Tract, Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Chailler","sequence":"additional","affiliation":[{"name":"Canadian Institute of Health Research Group on the Functional Development and Physiopathology of the Digestive Tract, Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"M\u00e9nard","sequence":"additional","affiliation":[{"name":"Canadian Institute of Health Research Group on the Functional Development and Physiopathology of the Digestive Tract, Department of Anatomy and Cell Biology, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(87)90848-1"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.99.2.407"},{"key":"B3","first-page":"31223","volume":"274","author":"Aplin AE","year":"1999","journal-title":"J Cell Biol"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1242\/dev.105.2.223"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4711"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(20000301)48:5<293::AID-JEMT6>3.0.CO;2-A"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115828"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1996.0180"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6336(97)80001-0"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.2741\/A429"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)43094-9"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.102.3.437"},{"key":"B13","first-page":"251","volume":"249","author":"Bissel MJ","year":"1987","journal-title":"Prog Clin Biol Res"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2000.18140"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1988.254.3.G355"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/s004290000104"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(99)00043-6"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3109\/15419069809004477"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7686"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1993.2"},{"key":"B21","first-page":"617","volume":"1","author":"Hafez MM","year":"1990","journal-title":"Cell Growth Differ"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(90)90821-H"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110101"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(97)00020-X"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.8.2013"},{"key":"B26","doi-asserted-by":"crossref","first-page":"5552","DOI":"10.4049\/jimmunol.163.10.5552","volume":"163","author":"Keates S","year":"1999","journal-title":"J Immunol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.2.595"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1038\/372739a0"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199812)177:4<618::AID-JCP12>3.0.CO;2-R"},{"key":"B30","first-page":"147","volume":"46","author":"M\u00e9nard D","year":"2001","journal-title":"Nestl\u00e9 Nutrition Workshop Series. 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