{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:24:38Z","timestamp":1790864678658,"version":"4.1.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2012,10,15]]},"abstract":"<jats:p> To define the stoichiometry and molecular identity of the Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchanger in the apical membrane of pancreatic duct cells, changes in luminal pH and volume were measured simultaneously in interlobular pancreatic ducts isolated from wild-type and Slc26a6-null mice. Transepithelial fluxes of HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup> were measured in the presence of anion gradients favoring rapid exchange of intracellular HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> with luminal Cl<jats:sup>\u2212<\/jats:sup> in cAMP-stimulated ducts. The flux ratio of Cl<jats:sup>\u2212<\/jats:sup> absorption\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> secretion was \u223c0.7 in wild-type ducts and \u223c1.4 in Slc26a6<jats:sup>\u2212\/\u2212<\/jats:sup> ducts where a different Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchanger, most likely SLC26A3, was found to be active. Interactions between Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchange and cystic fibrosis transmembrane conductance regulator (CFTR) in cAMP-stimulated ducts were examined by measuring the recovery of intracellular pH after alkali-loading by acetate prepulse. Hyperpolarization induced by luminal application of CFTRinh-172 enhanced HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> efflux across the apical membrane via SLC26A6 in wild-type ducts but significantly reduced HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> efflux in Slc26a6<jats:sup>\u2212\/\u2212<\/jats:sup> ducts. In microperfused wild-type ducts, removal of luminal Cl<jats:sup>\u2212<\/jats:sup>, or luminal application of dihydro-4,4\u2032-diisothiocyanatostilbene-2,2\u2032-disulphonic acid to inhibit SLC26A6, caused membrane hyperpolarization, which was abolished in Slc26a6<jats:sup>\u2212\/\u2212<\/jats:sup> ducts. In conclusion, we have demonstrated that deletion of Slc26a6 alters the apparent stoichiometry of apical Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchange in native pancreatic duct. Our results are consistent with SLC26A6 mediating 1:2 Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchange, and the exchanger upregulated in its absence, most probably SLC26A3, mediating 2:1 exchange. <\/jats:p>","DOI":"10.1152\/ajpcell.00151.2012","type":"journal-article","created":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T01:53:22Z","timestamp":1345082002000},"page":"C815-C824","source":"Crossref","is-referenced-by-count":21,"title":["Deletion of Slc26a6 alters the stoichiometry of apical Cl<sup>\u2212<\/sup>\/HCO<sub>3<\/sub><sup>\u2212<\/sup> exchange in mouse pancreatic duct"],"prefix":"10.1152","volume":"303","author":[{"given":"Ying","family":"Song","sequence":"first","affiliation":[{"name":"Laboratory of Human Nutrition, Nagoya University Graduate School of Medicine, Nagoya, Japan;"},{"name":"Second Clinical College of Norman Bethune Medical Division, Jilin University, Changchun, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Laboratory of Human Nutrition, Nagoya University Graduate School of Medicine, Nagoya, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin C.","family":"Steward","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru B. 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