{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:30:57Z","timestamp":1774315857472,"version":"3.50.1"},"reference-count":52,"publisher":"American Physiological Society","issue":"1","funder":[{"DOI":"10.13039\/501100000923","name":"Department of Education and Training | Australian Research Council","doi-asserted-by":"publisher","award":["DP140104165"],"award-info":[{"award-number":["DP140104165"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2023,1,1]]},"abstract":"<jats:p> Exercise training can increase both mitochondrial content and mitochondrial respiration. Despite its popularity, high-intensity exercise can be accompanied by mild acidosis (also present in certain pathological states), which may limit exercise-induced adaptations to skeletal muscle mitochondria. The aim of this study was to determine if administration of ammonium chloride (0.05 g\/kg) to Wistar rats before each individual exercise session (5 high-intensity exercise sessions\/wk for 8 wk) reduced training-induced increases in mitochondrial content (measured by citrate synthase activity and protein content of electron transport system complexes) and respiration (measured in permeabilized muscle fibers). In the soleus muscle, the exercise-training-induced increase in mitochondrial respiration was reduced in rats administered ammonium chloride compared to control animals, but mitochondrial content was not altered. These effects were not present in the white gastrocnemius muscle. In conclusion, ammonium chloride administration before each exercise session over 8 wk reduced improvements in mitochondrial respiration in the soleus muscle but did not alter mitochondrial content. This suggests that mild acidosis may affect training-induced improvements in the respiration of mitochondria in some muscles. <\/jats:p>","DOI":"10.1152\/ajpcell.00165.2022","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T13:20:44Z","timestamp":1669641644000},"page":"C67-C75","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Ammonium chloride administration prevents training-induced improvements in mitochondrial respiratory function in the soleus muscle of male rats"],"prefix":"10.1152","volume":"324","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7535-4071","authenticated-orcid":false,"given":"Amanda J.","family":"Genders","sequence":"first","affiliation":[{"name":"Institute for Health and Sport, Victoria University, Melbourne, Australia"},{"name":"Department of Biochemistry and Molecular Biology, Monash Biomedicine         Discovery Institute, Monash University, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jujiao","family":"Kuang","sequence":"additional","affiliation":[{"name":"Institute for Health and Sport, Victoria University, Melbourne, Australia"},{"name":"Australian Institute for Musculoskeletal Sciences, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Saner","sequence":"additional","affiliation":[{"name":"Institute for Health and Sport, Victoria University, Melbourne, Australia"},{"name":"Human Integrative Physiology, Baker Heart and Diabetes Institute, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9722-8519","authenticated-orcid":false,"given":"Javier","family":"Botella","sequence":"additional","affiliation":[{"name":"Institute for Health and Sport, Victoria University, Melbourne, Australia"},{"name":"Metabolic Research Unit, Institute for Mental and Physical Health and Clinical Translation (IMPACT), School of Medicine, Deakin University, Geelong, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6956-9188","authenticated-orcid":false,"given":"David J.","family":"Bishop","sequence":"additional","affiliation":[{"name":"Institute for Health and Sport, Victoria University, Melbourne, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.11.016"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.15-276907"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1007\/s40279-018-0936-y"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00625.2011"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01081.2012"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.02.035"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21186948"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2004.07.027"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2015.08.015"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1042\/CS20150838"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.199448"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00590.2006"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00738.2009"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00387.2003"},{"key":"B15","first-page":"19","volume":"59","author":"Sahlin K","year":"2008","journal-title":"J Physiol Pharmacol"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.045872"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118566"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004100842"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.5.1967"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00214.2018"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfi133"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0000000000000700"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0141317"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00137.2016"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1038\/nature10098"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00541-3"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13045"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1113\/JP272570"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.283267"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1976.tb10279.x"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1113\/JP276386"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-21-0242"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-382-0_3"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.03.013"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00038.2018"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.230185"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.85.4.1279"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01534.2005"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1984.56.4.831"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101733"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.10.012"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-021-00500-6"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27153-3"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1111\/sms.12136"},{"key":"B45","volume":"14","author":"Perez-Schindler J","year":"2009","journal-title":"Proc Physiol Soc"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14797"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2020.101110"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/832704"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.626117"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2078-2"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.115.1.201"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001710303"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00165.2022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T15:28:46Z","timestamp":1718638126000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00165.2022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,1]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1,1]]}},"alternative-id":["10.1152\/ajpcell.00165.2022"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00165.2022","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,1]]},"assertion":[{"value":"2022-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-21","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}