{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:21:51Z","timestamp":1778275311209,"version":"3.51.4"},"reference-count":42,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2003,3,1]]},"abstract":"<jats:p> Mutations in Na<jats:sup>+<\/jats:sup>-HCO[Formula: see text] cotransporter (NBC-1) cause proximal renal tubular acidosis (pRTA) associated with ocular abnormalities. One pRTA patient had increased serum amylase, suggesting possible evidence of pancreatitis. To further delineate a link between NBC-1 inactivation and pancreatic dysfunction, immunohistochemical analysis was performed on rat and human pancreas using antibodies against kidney-type (kNBC-1) and pancreatic-type (pNBC-1) transporters. In rat pancreas, the anti-pNBC-1 antibody labeled acinar cells and both apical and basolateral membranes of medium and large duct cells. In human pancreas, on the other hand, the anti-pNBC-1 antibody did not label acinar cells, although it did label the basolateral membranes of the entire duct system. The labeling by anti-kNBC-1 antibody was detected in only a limited number of rat pancreatic duct cells. To examine the effects of pRTA-related mutations, R342S and R554H, on pNBC-1 function, we performed functional analysis and found that both mutants had reduced transport activities compared with the wild-type pNBC-1. These results indicate that pNBC-1 is the predominant variant that mediates basolateral HCO[Formula: see text] uptake into duct cells in both rat and human pancreas. The loss of pNBC-1 function is predicted to have significant impact on overall ductal HCO[Formula: see text] secretion, which could potentially lead to pancreatic dysfunction. <\/jats:p>","DOI":"10.1152\/ajpcell.00166.2002","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:13:15Z","timestamp":1368490395000},"page":"C729-C737","source":"Crossref","is-referenced-by-count":60,"title":["Localization of Na<sup>+<\/sup>-HCO     3   \u2212    cotransporter (NBC-1)  variants in rat and human pancreas"],"prefix":"10.1152","volume":"284","author":[{"given":"Hiroaki","family":"Satoh","sequence":"first","affiliation":[{"name":"Departments of Internal Medicine,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Moriyama","sequence":"additional","affiliation":[{"name":"Hemodialysis and Apheresis, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiaki","family":"Hara","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideomi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Horita","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motoei","family":"Kunimi","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhisa","family":"Tsukamoto","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoyuki","family":"Iso-o","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Inatomi","sequence":"additional","affiliation":[{"name":"Pharmacology and Toxicology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Kudo","sequence":"additional","affiliation":[{"name":"Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Endou","sequence":"additional","affiliation":[{"name":"Pharmacology and Toxicology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Pediatrics, Faculty of Medicine, Tokyo University Tokyo 113-0033; 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