{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:46:47Z","timestamp":1790725607734,"version":"4.1.0"},"reference-count":47,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:p> During cell cycle progression, somatic cells exhibit different patterns of intracellular Ca<jats:sup>2+<\/jats:sup> signals during the G<jats:sub>0<\/jats:sub> phase, the transition from G<jats:sub>1<\/jats:sub> to S, and from G<jats:sub>2<\/jats:sub> to M. Because pluripotent embryonic stem (ES) cells progress through cell cycle without the gap phases G<jats:sub>1<\/jats:sub> and G<jats:sub>2<\/jats:sub>, we aimed to determine whether mouse ES (mES) cells still exhibit characteristic changes of intracellular Ca<jats:sup>2+<\/jats:sup> concentration during cell cycle progression. With confocal imaging of the Ca<jats:sup>2+<\/jats:sup>-sensitive dye fluo-4 AM, we identified that undifferentiated mES cells exhibit spontaneous Ca<jats:sup>2+<\/jats:sup> oscillations. In control cultures where 50.4% of the cells reside in the S phase of the cell cycle, oscillations appeared in 36% of the cells within a colony. Oscillations were not initiated by Ca<jats:sup>2+<\/jats:sup> influx but depended on inositol 1,4,5-trisphosphate (IP<jats:sub>3<\/jats:sub>)-mediated Ca<jats:sup>2+<\/jats:sup> release and the refilling of intracellular stores by a store-operated Ca<jats:sup>2+<\/jats:sup> influx (SOC) mechanism. Using cell cycle synchronization, we determined that Ca<jats:sup>2+<\/jats:sup> oscillations were confined to the G<jats:sub>1<\/jats:sub>\/S phase (\u223c70% oscillating cells vs. G<jats:sub>2<\/jats:sub>\/M with \u223c15% oscillating cells) of the cell cycle. ATP induced Ca<jats:sup>2+<\/jats:sup> oscillations, and activation of SOC could be induced in G<jats:sub>1<\/jats:sub>\/S and G<jats:sub>2<\/jats:sub>\/M synchronized cells. Intracellular Ca<jats:sup>2+<\/jats:sup> stores were not depleted, and all three IP<jats:sub>3<\/jats:sub> receptor isoforms were present throughout the cell cycle. Cell cycle analysis after EGTA, BAPTA-AM, 2-aminoethoxydiphenyl borate, thapsigargin, or U-73122 treatment emphasized that IP<jats:sub>3<\/jats:sub>-mediated Ca<jats:sup>2+<\/jats:sup> release is necessary for cell cycle progression through G<jats:sub>1<\/jats:sub>\/S. Because the IP<jats:sub>3<\/jats:sub> receptor sensitizer thimerosal induced Ca<jats:sup>2+<\/jats:sup> oscillations only in G<jats:sub>1<\/jats:sub>\/S, we propose that changes in IP<jats:sub>3<\/jats:sub> receptor sensitivity or basal levels of IP<jats:sub>3<\/jats:sub> could be the basis for the G<jats:sub>1<\/jats:sub>\/S-confined Ca<jats:sup>2+<\/jats:sup> oscillations. <\/jats:p>","DOI":"10.1152\/ajpcell.00181.2006","type":"journal-article","created":{"date-parts":[[2006,11,9]],"date-time":"2006-11-09T01:25:13Z","timestamp":1163035513000},"page":"C1510-C1518","source":"Crossref","is-referenced-by-count":91,"title":["Cell cycle-dependent calcium oscillations in mouse embryonic stem cells"],"prefix":"10.1152","volume":"292","author":[{"given":"Nidhi","family":"Kapur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Mignery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Banach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Banach K, Halbach MD, Hu P, Hescheler J, Egert U. 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