{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:23:26Z","timestamp":1790713406812,"version":"4.1.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,5,1]]},"abstract":"<jats:p> In fura 2-loaded N1E-115 cells, regulation of intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) following a Ca<jats:sup>2+<\/jats:sup> load induced by 1 \u03bcM thapsigargin and 10 \u03bcM carbonylcyanide p-trifluoromethyoxyphenylhydrazone (FCCP) was Na<jats:sup>+<\/jats:sup> dependent and inhibited by 5 mM Ni<jats:sup>2+<\/jats:sup>. In cells with normal intracellular Na<jats:sup>+<\/jats:sup> concentration ([Na<jats:sup>+<\/jats:sup>]<jats:sub>i<\/jats:sub>), removal of bath Na<jats:sup>+<\/jats:sup>, which should result in reversal of Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup>exchange, did not increase [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> unless cell Ca<jats:sup>2+<\/jats:sup> buffer capacity was reduced. When N1E-115 cells were Na<jats:sup>+<\/jats:sup> loaded using 100 \u03bcM veratridine and 4 \u03bcg\/ml scorpion venom, the rate of the reverse mode of the Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger was apparently enhanced, since an \u223c4- to 6-fold increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>occurred despite normal cell Ca<jats:sup>2+<\/jats:sup> buffering. In SBFI-loaded cells, we were able to demonstrate forward operation of the Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger (net efflux of Ca<jats:sup>2+<\/jats:sup>) by observing increases (\u223c 6 mM) in [Na<jats:sup>+<\/jats:sup>]<jats:sub>i<\/jats:sub>. These Ni<jats:sup>2+<\/jats:sup> (5 mM)-inhibited increases in [Na<jats:sup>+<\/jats:sup>]<jats:sub>i<\/jats:sub> could only be observed when a continuous ionomycin-induced influx of Ca<jats:sup>2+<\/jats:sup> occurred. The voltage-sensitive dye bis-(1,3-diethylthiobarbituric acid) trimethine oxonol was used to measure changes in membrane potential. Ionomycin (1 \u03bcM) depolarized N1E-115 cells (\u223c25 mV). This depolarization was Na<jats:sup>+<\/jats:sup>dependent and blocked by 5 mM Ni<jats:sup>2+<\/jats:sup> and 250\u2013500 \u03bcM benzamil. These data provide evidence for the presence of an electrogenic Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger that is capable of regulating [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> after release of Ca<jats:sup>2+<\/jats:sup> from cell stores. <\/jats:p>","DOI":"10.1152\/ajpcell.00182.2001","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:08:22Z","timestamp":1368490102000},"page":"C1000-C1008","source":"Crossref","is-referenced-by-count":9,"title":["Regulation of intracellular calcium in N1E-115 neuroblastoma cells: the role of Na<sup>+<\/sup>\/Ca<sup>2+<\/sup>exchange"],"prefix":"10.1152","volume":"282","author":[{"given":"Kara L.","family":"Kopper","sequence":"first","affiliation":[{"name":"Allergan, Inc., Department of Biological Sciences, Irvine, California 92612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph S.","family":"Adorante","sequence":"additional","affiliation":[{"name":"Allergan, Inc., Department of Biological Sciences, Irvine, California 92612"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.3.C721"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.47.030185.002553"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.3.763"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(77)85538-0"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.20.040180.000311"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.45.030183.001525"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76281-3"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.2741\/Garcia"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009210001608"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54285-X"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-05-03612.1995"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.84.4.565"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233458"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/BF01871102"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.397ae.x"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.106.5.975"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47321-3"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(95)90009-8"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012303"},{"key":"B22","first-page":"70","volume":"40","author":"Nuccitelli R.","year":"1994","journal-title":"Methods in Cell Biology."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)90479-0"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016797"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp016926"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(80)90243-6"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1177\/107385849600200311"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199801000-00002"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-02-00430.1992"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.2.7781924"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(98)90057-4"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.105.1.149"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.116.1.33"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.110.2.201"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00182.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:09:37Z","timestamp":1718651377000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00182.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,5,1]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,5,1]]}},"alternative-id":["10.1152\/ajpcell.00182.2001"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00182.2001","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,5,1]]}}}