{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T12:57:04Z","timestamp":1771678624631,"version":"3.50.1"},"reference-count":43,"publisher":"American Physiological Society","issue":"10","funder":[{"name":"NIH-NHLBI","award":["116776"],"award-info":[{"award-number":["116776"]}]},{"name":"NIH-NHLBI","award":["063887"],"award-info":[{"award-number":["063887"]}]},{"name":"NIH-NHLBI","award":["084207"],"award-info":[{"award-number":["084207"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2015,5,15]]},"abstract":"<jats:p> Endoplasmic reticulum (ER) stress and reactive oxygen species (ROS) generation in the brain circumventricular subfornical organ (SFO) mediate the central hypertensive actions of Angiotensin II (ANG II). However, the downstream signaling events remain unclear. Here we tested the hypothesis that angiotensin type 1a receptors (AT<jats:sub>1a<\/jats:sub>R), ER stress, and ROS induce activation of the transcription factor nuclear factor-\u03baB (NF-\u03baB) during ANG II-dependent hypertension. To spatiotemporally track NF-\u03baB activity in the SFO throughout the development of ANG II-dependent hypertension, we used SFO-targeted adenoviral delivery and longitudinal bioluminescence imaging in mice. During low-dose infusion of ANG II, bioluminescence imaging revealed a prehypertensive surge in NF-\u03baB activity in the SFO at a time point prior to a significant rise in arterial blood pressure. SFO-targeted ablation of AT<jats:sub>1a<\/jats:sub>R, inhibition of ER stress, or adenoviral scavenging of ROS in the SFO prevented the ANG II-induced increase in SFO NF-\u03baB. These findings highlight the utility of bioluminescence imaging to longitudinally track transcription factor activation during the development of ANG II-dependent hypertension and reveal an AT<jats:sub>1a<\/jats:sub>R-, ER stress-, and ROS-dependent prehypertensive surge in NF-\u03baB activity in the SFO. Furthermore, the increase in NF-\u03baB activity before a rise in arterial blood pressure suggests a causal role for SFO NF-\u03baB in the development of ANG II-dependent hypertension. <\/jats:p>","DOI":"10.1152\/ajpcell.00223.2014","type":"journal-article","created":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T03:02:12Z","timestamp":1426129332000},"page":"C803-C812","source":"Crossref","is-referenced-by-count":30,"title":["Endoplasmic reticulum and oxidant stress mediate nuclear factor-\u03baB activation in the subfornical organ during angiotensin II hypertension"],"prefix":"10.1152","volume":"308","author":[{"given":"Colin N.","family":"Young","sequence":"first","affiliation":[{"name":"Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anfei","family":"Li","sequence":"additional","affiliation":[{"name":"Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick N.","family":"Dong","sequence":"additional","affiliation":[{"name":"Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie A.","family":"Horwath","sequence":"additional","affiliation":[{"name":"Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catharine G.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Cornell University, Ithaca, New York; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin L.","family":"Davisson","sequence":"additional","affiliation":[{"name":"Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York;"},{"name":"Cell and Developmental Biology, Weill Cornell Medical College, New York, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s001090050360"},{"key":"B2","doi-asserted-by":"crossref","unstructured":"Brasier AR, Jamaluddin M, Han Y, Patterson C, Runge MS. 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