{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:18:37Z","timestamp":1790813917302,"version":"4.1.0"},"reference-count":34,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2018,10,1]]},"abstract":"<jats:p> We have recently demonstrated that whole egg ingestion induces a greater muscle protein synthetic (MPS) response when compared with isonitrogenous egg white ingestion after resistance exercise in young men. Our aim was to determine whether whole egg or egg white ingestion differentially influenced colocalization of key regulators of mechanistic target of rapamycin complex 1 (mTORC1) as means to explain our previously observed divergent postexercise MPS response. In crossover trials, 10 healthy resistance-trained men (21\u2009\u00b1\u20091 yr; 88\u2009\u00b1\u20093 kg; body fat: 16\u2009\u00b1\u20091%; means\u2009\u00b1\u2009SE) completed lower body resistance exercise before ingesting whole eggs (18 g protein, 17 g fat) or egg whites (18 g protein, 0 g fat). Muscle biopsies were obtained before exercise and at 120 and 300 min after egg ingestion to assess, by immunofluorescence, protein colocalization of key anabolic signaling molecules. After resistance exercise, tuberous sclerosis 2-Ras homolog enriched in brain (Rheb) colocalization decreased ( P &lt; 0.01) at 120 and 300 min after whole egg and egg white ingestion with concomitant increases ( P &lt; 0.01) in mTOR-Rheb colocalization. After resistance exercise, mTOR-lysosome-associated membrane protein 2 (LAMP2) colocalization significantly increased at 120 and 300 min only after whole egg ingestion ( P &lt; 0.01), and mTOR-LAMP2 colocalization correlated with rates of MPS at rest and after exercise ( r\u2009=\u20090.40, P &lt; 0.05). We demonstrated that the greater postexercise MPS response with whole egg ingestion is related in part to an enhanced recruitment of mTORC1-Rheb complexes to the lysosome during recovery. These data suggest nonprotein dietary factors influence the postexercise regulation of mRNA translation in human skeletal muscle. <\/jats:p>","DOI":"10.1152\/ajpcell.00225.2018","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T20:45:28Z","timestamp":1534970728000},"page":"C537-C543","source":"Crossref","is-referenced-by-count":35,"title":["Whole egg, but not egg white, ingestion induces mTOR colocalization with the lysosome after resistance exercise"],"prefix":"10.1152","volume":"315","author":[{"given":"Sidney","family":"Abou Sawan","sequence":"first","affiliation":[{"name":"Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"van Vliet","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Community Health, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W. D.","family":"West","sequence":"additional","affiliation":[{"name":"Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph W.","family":"Beals","sequence":"additional","affiliation":[{"name":"Division of Nutritional Sciences, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Paluska","sequence":"additional","affiliation":[{"name":"Department of Family Medicine, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Burd","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Community Health, University of Illinois, Urbana, Illinois"},{"name":"Division of Nutritional Sciences, University of Illinois, Urbana, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Moore","sequence":"additional","affiliation":[{"name":"Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13628"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201306041"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.114.103184"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1126\/science.aag1417"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0561927"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.106914"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3945\/jn.111.139485"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.163816"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00462.2010"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1249\/01.mss.0000210190.64458.25"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00512.2002"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00220-X"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.218255"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90411.2008"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00176.2017"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1110003"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.256339"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1186\/1743-7075-11-29"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01072.2013"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.772988"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2010.02187.x"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.26401"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.02.024"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1126\/science.1157535"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05483-x"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e31820751cb"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00076.2009"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(98)00087-4"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.117.159855"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.013722"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(94)00220-J"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(93)90302-G"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.531392"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1038\/ncb999"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00225.2018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T15:53:06Z","timestamp":1718639586000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00225.2018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,1]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,10,1]]}},"alternative-id":["10.1152\/ajpcell.00225.2018"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00225.2018","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,1]]}}}