{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:17:23Z","timestamp":1780939043475,"version":"3.54.1"},"reference-count":44,"publisher":"American Physiological Society","issue":"5","funder":[{"name":"NIH NIDDK","award":["DK104264"],"award-info":[{"award-number":["DK104264"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2019,11,1]]},"abstract":"<jats:p> The neonatal Fc receptor (FcRn) has been shown to be required for antigen presentation in dendritic cells, and global knockout of FcRn attenuates immune-mediated kidney disease. Podocytes express interleukin-6 (IL-6) receptor and produce IL-6 under proinflammatory conditions. Here we examined the role of FcRn in the IL-6-mediated inflammatory response in podocytes. We examined IL-6 production by ELISA and expression by qPCR in wild type (WT) and FcRn knockout (KO) podocytes after treatment with proinflammatory stimuli as well as IL-6-mediated signaling via the JAK\/STAT pathway. We also examined podocyte motility in cultured WT and KO podocytes after a proinflammatory challenge. We found that FcRn KO podocytes produced minimal amount of IL-6 after treatment with albumin, IgG, or immune complexes whereas WT podocytes had a robust response. FcRn KO podocytes also had minimal expression of IL-6 compared with WT. By Western blotting, there was significantly less phosphorylated STAT3 in KO podocytes after treatment with IFN\u03b3 or immune complexes. In a scratch assay, FcRn KO podocytes showed increased motility comparted KO, suggesting a defect in actin dynamics. Cultured FcRn KO podocytes also demonstrated abnormal stress fibers compared with WT and the defect could be rescued by IL-6 treatment. This study shows that in podocytes, FcRn modulates the IL-6 mediated response to proinflammatory stimuli and regulates podocytes actin structure, motility and synaptopodin expression. <\/jats:p>","DOI":"10.1152\/ajpcell.00235.2019","type":"journal-article","created":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T19:24:42Z","timestamp":1569439482000},"page":"C1048-C1060","source":"Crossref","is-referenced-by-count":11,"title":["Knockout of the neonatal Fc receptor in cultured podocytes alters IL-6 signaling and the actin cytoskeleton"],"prefix":"10.1152","volume":"317","author":[{"given":"Pantipa","family":"Tonsawan","sequence":"first","affiliation":[{"name":"Division of Renal Disease and Hypertension, University of Colorado School of Medicine, Aurora, Colorado"},{"name":"Division of Nephrology, Department of Medicine, Khon Kaen University, Khon Kaen, 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