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A-type currents have not been reported in the gastric fundus, a tonic smooth muscle. We used whole cell voltage clamp to identify and characterize A-type currents in smooth muscle cells (SMCs) isolated from murine fundus. A-type currents were robust in these cells with peak amplitudes averaging 1.5\u2009nA at 0\u2009mV. Inactivation was rapid with a time constant of 71\u2009ms at 0\u2009mV; recovery from inactivation at \u221280\u2009mV was similarly rapid with a time constant of 75\u2009ms. A-type currents in fundus were blocked by 4-aminopyridine (4-AP), flecainide, and phrixotoxin-1 (PaTX1). Remaining currents after 4-AP and PaTX1 displayed half-activation potentials that were shifted to more positive potentials and showed incomplete inactivation. Currents after tetraethylammonium (TEA) displayed half inactivation at \u221248.1\u2009\u00b1\u20091.0\u2009mV. Conventional microelectrode and contractile experiments on intact fundus muscles showed that 4-AP depolarized membrane potential and increased tone under conditions in which enteric neurotransmission was blocked. These data suggest that A-type K<jats:sup>+<\/jats:sup> channels in fundus SMCs are likely active at physiological membrane potentials, and sustained activation of A-type channels contributes to the negative membrane potentials of this tonic smooth muscle. Quantitative analysis of Kv4 expression showed that Kcnd3 was dominantly expressed in fundus SMCs. These data were confirmed by immunohistochemistry, which revealed Kv4.3-like immunoreactivity within the tunica muscularis. These observations indicate that Kv4 channels likely form the A-type current in murine fundus SMCs. <\/jats:p>","DOI":"10.1152\/ajpcell.00247.2021","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T19:46:46Z","timestamp":1629920806000},"page":"C684-C693","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of the A-type potassium current in murine gastric fundus smooth muscles"],"prefix":"10.1152","volume":"321","author":[{"given":"Gregory C.","family":"Amberg","sequence":"first","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Yeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, 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