{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T16:48:06Z","timestamp":1771519686599,"version":"3.50.1"},"reference-count":42,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2003,2,1]]},"abstract":"<jats:p> We examined the effects of metabolic inhibition on intracellular Ca<jats:sup>2+<\/jats:sup> release in single pulmonary arterial smooth muscle cells (PASMCs). Severe metabolic inhibition with cyanide (CN, 10 mM) increased intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and activated Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup> currents [I<jats:sub>Cl(Ca)<\/jats:sub>] in PASMCs, responses that were greatly inhibited by BAPTA-AM or caffeine. Mild metabolic inhibition with CN (1 mM) increased spontaneous transient inward currents and Ca<jats:sup>2+<\/jats:sup> sparks in PASMCs. In Xenopus oocytes, CN also induced Ca<jats:sup>2+<\/jats:sup> release and activated I<jats:sub>Cl(Ca)<\/jats:sub>, and these responses were inhibited by thapsigargin and cyclopiazonic acid to deplete sarcoplasmic reticulum (SR) Ca<jats:sup>2+<\/jats:sup>, whereas neither heparin nor anti-inositol 1,4,5-trisphosphate receptor (IP<jats:sub>3<\/jats:sub>R) antibodies affected CN responses. In both PASMCs and oocytes, CN-evoked Ca<jats:sup>2+<\/jats:sup>release was inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP) and oligomycin or CCCP and thapsigargin. Whereas hypoxic stimuli resulted in Ca<jats:sup>2+<\/jats:sup>release in pulmonary but not mesenteric artery myocytes, CN induced release in both cell types. We conclude that metabolic inhibition with CN increases [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in both pulmonary and systemic artery myocytes by stimulating Ca<jats:sup>2+<\/jats:sup> release from the SR and mitochondria. <\/jats:p>","DOI":"10.1152\/ajpcell.00260.2002","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:12:39Z","timestamp":1368490359000},"page":"C378-C388","source":"Crossref","is-referenced-by-count":43,"title":["Metabolic inhibition with cyanide induces calcium  release in pulmonary artery myocytes and <i>Xenopus<\/i> oocytes"],"prefix":"10.1152","volume":"284","author":[{"given":"Yong-Xiao","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Cardiovascular Sciences, Albany Medical College, Albany 12208; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Min","family":"Zheng","sequence":"additional","affiliation":[{"name":"Center for Cardiovascular Sciences, Albany Medical College, Albany 12208; 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