{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T12:19:11Z","timestamp":1779279551649,"version":"3.51.4"},"reference-count":92,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p> Cochlear endolymph has a highly positive potential of approximately +80 mV known as the endocochlear potential (EP). The EP is essential for hearing and is maintained by K<jats:sup>+<\/jats:sup> circulation from perilymph to endolymph through the cochlear lateral wall. Various K<jats:sup>+<\/jats:sup> transport apparatuses such as the Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>-ATPase, the Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup> cotransporter, and the K<jats:sup>+<\/jats:sup> channels Kir4.1 and KCNQ1\/KCNE1 are expressed in the lateral wall and are known to play indispensable roles in cochlear K<jats:sup>+<\/jats:sup> circulation. The gastric type of the H<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>-ATPase was also shown to be expressed in the cochlear lateral wall (Lecain E, Robert JC, Thomas A, and Tran Ba Huy P. Hear Res 149: 147\u2013154, 2000), but its functional role has not been well studied. In this study we examined the precise localization of H<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>-ATPase in the cochlea and its involvement in formation of EP. RT-PCR analysis showed that the cochlea expressed mRNAs of gastric \u03b1<jats:sub>1<\/jats:sub>-, but not colonic \u03b1<jats:sub>2<\/jats:sub>-, and \u03b2-subunits of H<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>-ATPase. Immunolabeling of an antibody specific to the \u03b1<jats:sub>1<\/jats:sub> subunit was detected in type II, IV, and V fibrocytes distributed in the spiral ligament of the lateral wall and in the spiral limbus. Strong immunoreactivity was also found in the stria vascularis. Immunoelectron microscopic examination exhibited that the H<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>-ATPase was localized exclusively at the basolateral site of strial marginal cells. Application of Sch-28080, a specific inhibitor of gastric H<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>-ATPase, to the spiral ligament as well as to the stria vascularis caused prominent reduction of EP. These results may imply that the H<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>-ATPase in the cochlear lateral wall is crucial for K<jats:sup>+<\/jats:sup> circulation and thus plays a critical role in generation of EP. <\/jats:p>","DOI":"10.1152\/ajpcell.00266.2006","type":"journal-article","created":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T08:54:02Z","timestamp":1152176042000},"page":"C1038-C1048","source":"Crossref","is-referenced-by-count":45,"title":["Gastric type H<sup>+<\/sup>,K<sup>+<\/sup>-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential"],"prefix":"10.1152","volume":"291","author":[{"given":"Toshiaki","family":"Shibata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Hibino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Doi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Hisa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihisa","family":"Kurachi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Ando M and Takeuchi S. 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