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Few BK<jats:sub>Ca<\/jats:sub>channel openers have been accepted for clinical development. The effect of the novel BK<jats:sub>Ca<\/jats:sub>channel opener GoSlo-SR5-130 on electrical activity in isolated rabbit CCSM cells and mechanical activity in strips of rabbit CCSM was examined. Single-channel currents were observed in inside-out patches. These channels were sensitive to Ca<jats:sup>2+<\/jats:sup>, blocked by penitrem A, and had a conductance of 291 \u00b1 20 pS ( n = 7). In the presence of GoSlo-SR5-130, the number of open BK<jats:sub>Ca<\/jats:sub>channels increased. Using voltage-ramp protocols, GoSlo-SR5-130 caused currents to activate at more negative potentials in a concentration-dependent manner, shifting the half-maximal activation voltage potential to the left on the voltage axis. Therefore, BK<jats:sub>Ca<\/jats:sub>channels were open within the physiological range of membrane potentials in the presence of GoSlo-SR5-130. GoSlo-SR5-130 also resulted in an increase in the activity of spontaneous transient outward currents in myocytes isolated from CCSM, and this effect was reversed by iberiotoxin. In current-clamp mode, GoSlo-SR5-130 hyperpolarized the cell membrane. Isometric tension recording of strips of rabbit corpus cavernosum showed that GoSlo-SR5-130 inhibited spontaneous contractions in a concentration-dependent manner. This effect was reversed in the presence of iberiotoxin, suggesting that GoSlo-SR5-130 exerts its effect through BK<jats:sub>Ca<\/jats:sub>channels. These findings suggest that GoSlo-SR5-130 is an effective tool for the study of BK<jats:sub>Ca<\/jats:sub>channels and that these channels can modulate CCSM activity and are possible targets for the treatment of erectile dysfunction.<\/jats:p>","DOI":"10.1152\/ajpcell.00273.2015","type":"journal-article","created":{"date-parts":[[2015,12,10]],"date-time":"2015-12-10T04:54:54Z","timestamp":1449723294000},"page":"C284-C292","source":"Crossref","is-referenced-by-count":14,"title":["Effect of a novel BK<sub>Ca<\/sub>opener on BK<sub>Ca<\/sub>currents and contractility of the rabbit corpus cavernosum"],"prefix":"10.1152","volume":"310","author":[{"given":"K. I.","family":"Hannigan","sequence":"first","affiliation":[{"name":"Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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