{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T04:36:40Z","timestamp":1769575000782,"version":"3.49.0"},"reference-count":36,"publisher":"American Physiological Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2013,12,1]]},"abstract":"<jats:p> Infections with enteric pathogens like enterotoxigenic Escherichia coli ( ETEC) is a major health issue worldwide and while diarrhea is the major problem, prolonged, severe, and dual infections with multiple pathogens may also compromise the nutritional status of the infected individuals. There is almost nothing currently known about the effect of ETEC infection on intestinal absorptions of water-soluble vitamins including thiamin. We examined the effect of ETEC infection on intestinal uptake of the thiamin using as a model the human-derived intestinal epithelial Caco-2 cells. The results showed that infecting confluent Caco-2 monolayers with live ETEC (but not with boiled\/killed ETEC or nonpathogenic E. coli) or treatment with bacterial culture supernatant led to a significant inhibition in thiamin uptake. This inhibition appears to be caused by a heat-labile and -secreted ETEC component and is mediated via activation of the epithelial adenylate cyclase system. The inhibition in thiamin uptake by ETEC was associated with a significant reduction in expression of human thiamin transporter-1 and -2 (hTHTR1 and hTHTR2) at the protein and mRNA levels as well as in the activity of the SLC19A2 and SLC19A3 promoters. Dual infection of Caco-2 cells with ETEC and EPEC (enteropathogenic E. coli) led to compounded inhibition in intestinal thiamin uptake. These results show for the first time that infection of human intestinal epithelial cells with ETEC causes a significant inhibition in intestinal thiamin uptake. This inhibition is mediated by a secreted heat-labile toxin and is associated with a decrease in the expression of intestinal thiamin transporters. <\/jats:p>","DOI":"10.1152\/ajpcell.00276.2013","type":"journal-article","created":{"date-parts":[[2013,10,18]],"date-time":"2013-10-18T06:12:03Z","timestamp":1382076723000},"page":"C1185-C1191","source":"Crossref","is-referenced-by-count":15,"title":["Enterotoxigenic <i>Escherichia coli<\/i> infection and intestinal thiamin uptake: studies with intestinal epithelial Caco-2 monolayers"],"prefix":"10.1152","volume":"305","author":[{"given":"Abhisek","family":"Ghosal","sequence":"first","affiliation":[{"name":"Departments of Medicine and Physiology\/Biophysics, University of California, School of Medicine, Irvine, California and Medical Research Service, Veterans Affairs Medical Center, Long Beach, California; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabendu S.","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology\/Biophysics, University of California, School of Medicine, Irvine, California and Medical Research Service, Veterans Affairs Medical Center, Long Beach, California; and"},{"name":"National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tristan","family":"Chou","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology\/Biophysics, University of California, School of Medicine, Irvine, California and Medical Research Service, Veterans Affairs Medical Center, Long Beach, California; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid M.","family":"Said","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology\/Biophysics, University of California, School of Medicine, Irvine, California and Medical Research Service, Veterans Affairs Medical Center, Long Beach, California; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1080\/02724936.1985.11748353"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00250.2009"},{"key":"B3","doi-asserted-by":"crossref","unstructured":"Berdanier CD. 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