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Treatment of acini with the serine\/threonine phosphatase inhibitor calyculin A blocked both thapsigargin- and carbachol-induced Ca<jats:sup>2+<\/jats:sup>entry but resulted in an enhancement of AA-induced Ca<jats:sup>2+<\/jats:sup>release and entry. Effects were mimicked by the protein phosphatase-1 (PP1) inhibitor tautomycin but were inhibited by the PP2A inhibitor okadaic acid. The protein kinase A (PKA) inhibitor PKI<jats:sub>(14-22)<\/jats:sub>significantly attenuated AA-induced enhancement of Ca<jats:sup>2+<\/jats:sup>release and entry in the presence of calyculin A, whereas it had no effect on calyculin A-induced inhibition of thapsigargin-induced Ca<jats:sup>2+<\/jats:sup>responses. The ryanodine receptor (RyR) inhibitor, tetracaine, and StHt-31, a peptide known to competitively inhibit type II PKA regulatory subunit binding to PKA-anchoring protein (AKAP), abolished calyculin A enhancement of AA-induced Ca<jats:sup>2+<\/jats:sup>release and entry. StHt-31 also abolished forskolin potentiation of 4-chloro-3-ethylphenol (4-CEP) and AA on Ca<jats:sup>2+<\/jats:sup>release but had no effect on 8-(4-methoxyphenylthio)-2\u2032- O-methyladenosine-3\u2032,5\u2032-cAMP potentiation of 4-CEP responses. Results suggest that inhibition of PP1 results in an enhancement of AA-induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>via PKA, AKAP, and RyRs.<\/jats:p>","DOI":"10.1152\/ajpcell.00281.2008","type":"journal-article","created":{"date-parts":[[2008,11,6]],"date-time":"2008-11-06T01:54:56Z","timestamp":1225936496000},"page":"C88-C96","source":"Crossref","is-referenced-by-count":11,"title":["Inhibition of serine\/threonine phosphatase enhances arachidonic acid-induced [Ca<sup>2+<\/sup>]<sub>i<\/sub>via protein kinase A"],"prefix":"10.1152","volume":"296","author":[{"given":"Tomoyuki","family":"Saino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eileen L.","family":"Watson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Bojesen IN, Bojesen E.Binding of arachidonate and oleate to bovine serum albumin.J Lipid Res35: 770\u2013778, 1994.","DOI":"10.1016\/S0022-2275(20)39172-0"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Broad LM, Cannon TR, Taylor CW.A non-capacitative pathway activated by arachidonic acid is the major Ca2+entry mechanism in rat A7r5 smooth muscle cells stimulated with low concentrations of vasopressin.J Physiol517: 121\u2013134, 1999.","DOI":"10.1111\/j.1469-7793.1999.0121z.x"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Bruce JI, Shuttleworth TJ, Giovannucci DR, Yule DI.Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. 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