{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:35:46Z","timestamp":1790861746749,"version":"4.1.0"},"reference-count":32,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,12,1]]},"abstract":"<jats:p> The ductal epithelium of the semicircular canal forms much of the boundary between the K<jats:sup>+<\/jats:sup>-rich luminal fluid and the Na<jats:sup>+<\/jats:sup>-rich abluminal fluid. We sought to determine whether the net ion flux producing the apical-to-basal short-circuit current ( I <jats:sub>sc<\/jats:sub>) in primary cultures was due to anion secretion and\/or cation absorption and under control of receptor agonists. Net fluxes of <jats:sup>22<\/jats:sup>Na, <jats:sup>86<\/jats:sup>Rb, and<jats:sup>36<\/jats:sup>Cl demonstrated a basal-to-apical Cl<jats:sup>\u2212<\/jats:sup>secretion that was stimulated by isoproterenol. Isoproterenol and norepinephrine increased I <jats:sub>sc<\/jats:sub> with an EC<jats:sub>50<\/jats:sub> of 3 and 15 nM, respectively, and isoproterenol increased tissue cAMP of native canals with an EC<jats:sub>50<\/jats:sub> of 5 nM. Agonists for adenosine, histamine, and vasopressin receptors had no effect on I <jats:sub>sc<\/jats:sub>. Isoproterenol stimulation of I <jats:sub>sc<\/jats:sub> and cAMP was inhibited by ICI-118551 (IC<jats:sub>50<\/jats:sub> = 6 \u03bcM for I <jats:sub>sc<\/jats:sub>) but not by CGP-20712A (1 \u03bcM) in primary cultures, and similar results were found in native epithelium. I <jats:sub>sc<\/jats:sub> was partially inhibited by basolateral Ba<jats:sup>2+<\/jats:sup> (IC<jats:sub>50<\/jats:sub> = 0.27 mM) and ouabain, whereas responses to genistein, glibenclamide, and DIDS did not fully fit the profile for CFTR. Our findings show that the canal epithelium contributes to endolymph homeostasis by secretion of Cl<jats:sup>\u2212<\/jats:sup> under \u03b2<jats:sub>2<\/jats:sub>-adrenergic control with cAMP as second messenger, a process that parallels the adrenergic control of K<jats:sup>+<\/jats:sup> secretion by vestibular dark cells. The current work points to one possible etiology of endolymphatic hydrops in Meniere's disease and may provide a basis for intervention. <\/jats:p>","DOI":"10.1152\/ajpcell.00283.2002","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:11:39Z","timestamp":1368490299000},"page":"C1752-C1760","source":"Crossref","is-referenced-by-count":35,"title":["Chloride secretion by semicircular canal duct epithelium  is stimulated via \u03b2<sub>2<\/sub>-adrenergic receptors"],"prefix":"10.1152","volume":"283","author":[{"given":"Pierre G.","family":"Milhaud","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Unit\u00e9 432 Vestibular Neurobiology, Universit\u00e9 Montpellier II, 34095 Montpellier, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satyanarayana R.","family":"Pondugula","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Herzog","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Lehouelleur","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Unit\u00e9 432 Vestibular Neurobiology, Universit\u00e9 Montpellier II, 34095 Montpellier, France; 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