{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:57:49Z","timestamp":1792810669763,"version":"4.5.0"},"reference-count":72,"publisher":"American Physiological Society","issue":"6","funder":[{"DOI":"10.13039\/100017540","name":"HHS | NIH | NHLBI | Division of Intramural Research","doi-asserted-by":"publisher","award":["HL155786"],"award-info":[{"award-number":["HL155786"]}],"id":[{"id":"10.13039\/100017540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017540","name":"HHS | NIH | NHLBI | Division of Intramural Research","doi-asserted-by":"publisher","award":["HL142568"],"award-info":[{"award-number":["HL142568"]}],"id":[{"id":"10.13039\/100017540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002590","name":"American Lung Association","doi-asserted-by":"publisher","award":["HIA-1278193"],"award-info":[{"award-number":["HIA-1278193"]}],"id":[{"id":"10.13039\/100002590","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2025,6,1]]},"abstract":"<jats:p>Stromal cells in the lung provide structural support to other cells and play critical roles in inflammation, repair, and fibrosis after injury. Recent technological advancements in single-cell genomics have tremendously improved our knowledge of stromal heterogeneity in the lung. Stromal heterogeneity in single-cell RNA sequencing data is often conserved across different studies despite the different annotation strategies. Spatial analyses suggest that each stromal subset is characterized by unique anatomic locations in the adult lung. This review overviews the stromal heterogeneity delineated by single-cell RNA sequencing studies and highlights the functional characteristics and locations of each population.<\/jats:p>","DOI":"10.1152\/ajpcell.00285.2025","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T07:01:38Z","timestamp":1747033298000},"page":"C1964-C1972","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Stromal heterogeneity in the adult lung delineated by single-cell genomics"],"prefix":"10.1152","volume":"328","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3100-6934","authenticated-orcid":false,"given":"Tatsuya","family":"Tsukui","sequence":"first","affiliation":[{"name":"Division of Pulmonary, Critical Care, Allergy and Sleep, Department of Medicine","place":["United States"]},{"name":"University of California","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6277-2036","authenticated-orcid":false,"given":"Dean","family":"Sheppard","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care, Allergy and Sleep, Department of Medicine","place":["United States"]},{"name":"University of California","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI68782"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20210815"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2877-5"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07660-1"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05272-1"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2014.0118"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21889-7"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.04.005"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.03.010"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.07.034"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15647-5"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.227900"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00350.2015"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2018-0313OC"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-025-02080-x"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/acel.14503"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2615"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1172\/JCI132189"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/nature12930"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1006\/mvre.1995.1067"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200303-371OC"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam6603"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2015-0095OC"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08542-2"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2020-0095MA"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1242\/dev.202659"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2922-4"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.141618"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04541-3"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00254.2018"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.10.004"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.59.1.43"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba1972"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba1983"},{"key":"B35","first-page":"865","volume":"67","author":"Michel RP","year":"1986","journal-title":"Br J Exp Pathol"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c090017"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.02.002"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1242\/dev.200081"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03549-5"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-022-01243-4"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.07.028"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1242\/dev.181032"},{"key":"B43","volume-title":"Impaired Myofibroblast Proliferation Is a Central Feature of Pathologic Post-Natal Alveolar Simplification","author":"Khan IS","year":"2024"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1242\/dev.202430"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.01.032"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00259.2013"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111863"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1113\/JP270033"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2007.07.006"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.120.045750"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102551"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.09.015"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117988108"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04913-2"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201212-2297OC"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00349.2016"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1038\/s44318-024-00349-1"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01326-2023"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.172977"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-07589-9"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2023.10.001"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1126\/science.ado5561"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-214865"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2024.08.020"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03569-1"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.115248"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2022-0292OC"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-019-0324-5"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00482-2023"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1016\/j.medj.2022.05.002"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02327-2"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40173-5"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00285.2025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T12:18:17Z","timestamp":1749644297000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00285.2025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,1]]},"references-count":72,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6,1]]}},"alternative-id":["10.1152\/ajpcell.00285.2025"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00285.2025","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,1]]},"assertion":[{"value":"2025-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-11","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}