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The mechanisms through which ATP and BzATP increase [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in CGCs are unclear. Ryanodine receptors (RyRs) are fundamental in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> regulation in excitable cells. Herein, we find that ATP and BzATP increase [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> through the activation of protein kinase A, voltage-gated calcium channels, and RyRs, and that RyRs are crucial for nonexcitable CGCs\u2019 Ca<jats:sup>2+<\/jats:sup><jats:sub>i<\/jats:sub> homeostasis. <\/jats:p>","DOI":"10.1152\/ajpcell.00291.2024","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T08:01:53Z","timestamp":1722844913000},"page":"C830-C843","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Purinergic agonists increase [Ca<sup>2+<\/sup>]<sub>i<\/sub> in rat conjunctival goblet cells through ryanodine receptor type 3"],"prefix":"10.1152","volume":"327","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1945-4742","authenticated-orcid":false,"given":"Haakon K.","family":"Fjaervoll","sequence":"first","affiliation":[{"name":"Division of Head, Neck and Reconstructive 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