{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:22:56Z","timestamp":1791285776458,"version":"4.1.0"},"reference-count":48,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p> Adipokines may represent a mechanism linking insulin resistance to cardiovascular disease. We showed previously that homocysteine (Hcy), an independent risk factor for cardiovascular disease, can induce the expression and secretion of resistin, a novel adipokine, in vivo and in vitro. Since vascular smooth muscle cell (VSMC) migration is a key event in vascular disease, we hypothesized that adipocyte-derived resistin is involved in Hcy-induced VSMC migration. To confirm our hypothesis, Sprague-Dawley rat aortic SMCs were cocultured with Hcy-stimulated primary rat epididymal adipocytes or treated directly with increasing concentrations of resistin for up to 24 h. Migration of VSMCs was investigated. Cytoskeletal structure and cytoskeleton-related proteins were also detected. The results showed that Hcy (300\u2013500 \u03bcM) increased migration significantly in VSMCs cocultured with adipocytes but not in VSMC cultured alone. Resistin alone also significantly increased VSMC migration in a time- and concentration-dependent manner. Resistin small interfering RNA (siRNA) significantly attenuated VSMC migration in the coculture system, which indicated that adipocyte-derived resistin mediates Hcy-induced VSMC migration. On cell spreading assay, resistin induced the formation of focal adhesions near the plasma membrane, which suggests cytoskeletal rearrangement via an \u03b1<jats:sub>5<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>-integrin-focal adhesion kinase\/paxillin-Ras-related C3 botulinum toxin substrate 1 (Rac1) pathway. Our data demonstrate that Hcy promotes VSMC migration through a paracrine or endocrine effect of adipocyte-derived resistin, which provides further evidence of the adipose-vascular interaction in metabolic disorders. The migratory action exerted by resistin on VSMCs may account in part for the increased incidence of restenosis in diabetic patients. <\/jats:p>","DOI":"10.1152\/ajpcell.00304.2009","type":"journal-article","created":{"date-parts":[[2009,10,15]],"date-time":"2009-10-15T02:12:36Z","timestamp":1255572756000},"page":"C1466-C1476","source":"Crossref","is-referenced-by-count":53,"title":["Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin"],"prefix":"10.1152","volume":"297","author":[{"given":"Changtao","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Physiology and Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology Embryology, School of Basic Medical Sciences, Peking University,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingbo","family":"Xu","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, King's College London British Heart Foundation Centre, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology and"},{"name":"Institute of Vascular Medicine, Peking University Third Hospital, and"},{"name":"Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, People's Republic of China; 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