{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:26:34Z","timestamp":1760984794948},"reference-count":39,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,8,1]]},"abstract":"<jats:p> We examined the influence of zinc status on expression of certain transcription factors involved in regulation of apolipoprotein A-I (apoAI) expression in human hepatoblastoma Hep G2 cells. A low zinc basal medium (zinc deficient, ZD) consisting of DMEM and 10% Chelex100-treated fetal bovine serum was used to deplete cellular zinc over one passage. Cells were also cultured for one passage in medium supplemented with 0.4 (ZD0.4), 4.0 (zinc normal, ZN), 16.0 (zinc adequate, ZA), or 32.0 \u03bcM zinc (zinc supplemented, ZS). Compared with ZN cells, cellular zinc levels were 43 and 31% lower in ZD and ZD0.4 cells but 70 and 146% higher in ZA and ZS cells, respectively. Supplementation of 0.4 \u03bcM zinc significantly increased DNA contents per plate, from 65% in ZD cells to 83% in ZD0.4 cells compared with ZN cells. Addition of &gt;4 \u03bcM zinc in medium did not further increase DNA contents. The proportion of cells in G<jats:sub>1<\/jats:sub>\/S and S phase was about fourfold higher and threefold lower, respectively, in ZD cells compared with ZN and other groups. Nuclear Egr-1 protein was markedly decreased in ZD and ZD0.4 cells. Moreover, hepatocyte nuclear factor (HNF)-3\u03b2 was severely degraded in ZD and ZD0.4 cells. In contrast, HNF-4\u03b1 remained stable in all groups and was not significantly lower in ZD and ZD0.4 cells. Furthermore, downregulation of trans-acting factor Egr-1 and cleavage of HNF-3\u03b2 were associated with reduction of apoAI promoter activity in zinc-deficient Hep G2 cells. Thus zinc is critical in transcriptional regulation of apoAI gene expression in hepatocytes. <\/jats:p>","DOI":"10.1152\/ajpcell.00308.2001","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:09:41Z","timestamp":1368490181000},"page":"C623-C630","source":"Crossref","is-referenced-by-count":19,"title":["Zinc depletion reduced Egr-1 and HNF-3\u03b2 expression  and apolipoprotein A-I promoter activity in Hep G2 cells"],"prefix":"10.1152","volume":"283","author":[{"given":"Libin","family":"Cui","sequence":"first","affiliation":[{"name":"Department of Nutrition and Food Science, University of Maryland, College Park, 20742; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norberta W.","family":"Schoene","sequence":"additional","affiliation":[{"name":"Nutrient Requirements and Functions Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Science, University of Maryland, College Park, 20742; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica C.","family":"Fanzo","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Science, University of Maryland, College Park, 20742; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Alshatwi","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Science, University of Maryland, College Park, 20742; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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