{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:18:17Z","timestamp":1790014697815,"version":"4.0.1"},"reference-count":48,"publisher":"American Physiological Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:p> Astrocytes, the most numerous cells in the human brain, play a central role in the metabolic homeostasis following hypoxic injury. Caveolin-1 (Cav-1), a transmembrane scaffolding protein, has been shown to converge prosurvival signaling in the central nerve system. The present study aimed to investigate the role of Cav-1 in the hypoxia-induced astrocyte injury. We also examined how Cav-1 alleviates apoptotic astrocyte death. To this end, primary astrocytes were exposed to oxygen-glucose deprivation (OGD) for 6 h and a subsequent 24-h reoxygenation to mimic hypoxic injury. OGD significantly reduced Cav-1 expression. Downregulation of Cav-1 using Cav-1 small interfering RNA dramatically worsened astrocyte cell damage and impaired cellular glutamate uptake after OGD, whereas overexpression of Cav-1 with Cav-1 scaffolding domain peptide attenuated OGD-induced cell apoptosis. Mechanistically, the expressions of Ras-GTP, phospho-Raf, and phospho-ERK were sequestered in Cav-1 small interfering RNA-treated astrocytes, yet were stimulated after supplementation with caveolin peptide. MEK\/ERK inhibitor U0126 remarkably blocked the Cav-1-induced counteraction against apoptosis following hypoxia, indicating Ras\/Raf\/ERK pathway is required for the Cav-1's prosurvival role. Together, these findings support Cav-1 as a checkpoint for the in hypoxia-induced astrocyte apoptosis and warrant further studies targeting Cav-1 to treat hypoxic-ischemic brain injury. <\/jats:p>","DOI":"10.1152\/ajpcell.00309.2015","type":"journal-article","created":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T04:49:57Z","timestamp":1458794997000},"page":"C903-C910","source":"Crossref","is-referenced-by-count":39,"title":["Caveolin-1 is a checkpoint regulator in hypoxia-induced astrocyte apoptosis via Ras\/Raf\/ERK pathway"],"prefix":"10.1152","volume":"310","author":[{"given":"Lili","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Neurology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu Province, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liumin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Jinling Hospital, Medical School of Nanjing University, Nanjing, 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