{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T03:42:04Z","timestamp":1790480524808,"version":"4.1.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p>\n                    Aldosterone classically modulates Na transport in tight epithelia such as the renal collecting duct (CD) through the transcellular route, but it is not known whether the hormone could also affect paracellular permeability. Such permeability is controlled by tight junctions (TJ) that form a size- and charge-selective barrier. Among TJ proteins, claudin-4 has been highlighted as a key element to control paracellular charge selectivity. In RCCD2 CD cells grown on filters, we have identified novel early aldosterone effects on TJ. Endogenous claudin-4 abundance and cellular localization were unaltered by aldosterone. However, the hormone promoted rapid (within 15\u201320 min) and transient phosphorylation of endogenous claudin-4 on threonine residues, without affecting tyrosine or serine; this event was fully developed at 10 nM aldosterone and appeared specific for aldosterone (because it is not observed after dexamethasone treatment and it depends on mineralocorticoid receptor occupancy). Within the same delay, aldosterone also promoted an increased apical-to-basal passage of\n                    <jats:sup>125<\/jats:sup>\n                    I (a substitute for\n                    <jats:sup>36<\/jats:sup>\n                    Cl), whereas\n                    <jats:sup>22<\/jats:sup>\n                    Na passage was unaffected; paracellular permeability to [\n                    <jats:sup>3<\/jats:sup>\n                    H]mannitol was also reduced. Later on (45 min), a fall in transepithelial resistance was observed. These data indicate that aldosterone modulates TJ properties in renal epithelial cells.\n                  <\/jats:p>","DOI":"10.1152\/ajpcell.00314.2005","type":"journal-article","created":{"date-parts":[[2005,8,17]],"date-time":"2005-08-17T20:24:57Z","timestamp":1124310297000},"page":"C1513-C1521","source":"Crossref","is-referenced-by-count":91,"title":["Aldosterone and tight junctions: modulation of claudin-4 phosphorylation in renal collecting duct cells"],"prefix":"10.1152","volume":"289","author":[{"given":"Cathy","family":"Le Moellic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheerazed","family":"Boulkroun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Gonz\u00e1lez-Nunez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Dublineau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francoise","family":"Cluzeaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Fay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Blot-Chabaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolette","family":"Farman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Anderson 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WY\n                      , Huey CL, and Yu AS.\n                      Expression of claudin-7 and -8 along the mouse nephron.\n                      Am J Physiol Renal Physiol\n                      286\n                      : F1063\u2013F1071, 2004.","DOI":"10.1152\/ajprenal.00384.2003"},{"key":"R20","doi-asserted-by":"crossref","unstructured":"Matter K\n                      and Balda MS.\n                      Signalling to and from tight junctions.\n                      Nat Rev Mol Cell Biol\n                      4\n                      : 225\u2013236, 2003.","DOI":"10.1038\/nrm1055"},{"key":"R21","unstructured":"Morris RG\n                      and Knepper MA.\n                      Aldosterone infusion increases WNK4 abundance in mouse kidney.\n                      J Am Soc Nephrol\n                      15\n                      : 307A, 2004."},{"key":"R22","doi-asserted-by":"crossref","unstructured":"N\u00e1ray-Fejes-T\u00f3th A\n                      , Snyder PM, and Fejes-T\u00f3th G.\n                      The kidney-specific WNK1 isoform is induced by aldosterone and stimulates epithelial sodium channel-mediated Na\n                      +\n                      transport.\n                      Proc Natl Acad Sci USA\n                      101\n                      : 17434\u201317439, 2004.","DOI":"10.1073\/pnas.0408146101"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Paccolat MP\n                      , Geering K, Gaeggeler HP, and Rossier BC.\n                      Aldosterone regulation of Na\n                      +\n                      transport and Na\n                      +\n                      -K\n                      +\n                      -ATPase in A6 cells: role of growth conditions.\n                      Am J Physiol Cell Physiol\n                      252\n                      : C468\u2013C476, 1987.","DOI":"10.1152\/ajpcell.1987.252.5.C468"},{"key":"R24","doi-asserted-by":"crossref","unstructured":"Pearce D\n                      , Bhargava A, and Cole TJ.\n                      Aldosterone: its receptor, target genes, and actions.\n                      Vitam Horm\n                      66\n                      : 29\u201376, 2003.","DOI":"10.1016\/S0083-6729(03)01002-1"},{"key":"R25","doi-asserted-by":"crossref","unstructured":"Sansom SC\n                      and O'Neil RG.\n                      Mineralocorticoid regulation of apical cell membrane Na\n                      +\n                      and K\n                      +\n                      transport of the cortical collecting duct.\n                      Am J Physiol Renal Fluid Electrolyte Physiol\n                      248\n                      : F858\u2013F868, 1985.","DOI":"10.1152\/ajprenal.1985.248.6.F858"},{"key":"R26","doi-asserted-by":"crossref","unstructured":"Schuster VL\n                      and Stokes JB.\n                      Chloride transport by the cortical and outer medullary collecting duct.\n        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1998.","DOI":"10.1007\/s002329900321"},{"key":"R31","doi-asserted-by":"crossref","unstructured":"Tsukamoto T\n                      and Nigam SK.\n                      Role of tyrosine phosphorylation in the reassembly of occludin and other tight junction proteins.\n                      Am J Physiol Renal Physiol\n                      276\n                      : F737\u2013F750, 1999.","DOI":"10.1152\/ajprenal.1999.276.5.F737"},{"key":"R32","doi-asserted-by":"crossref","unstructured":"Tsukita S\n                      , Furuse M, and Itoh M.\n                      Multifunctional strands in tight junctions.\n                      Nat Rev Mol Cell Biol\n                      2\n                      : 285\u2013293, 2001.","DOI":"10.1038\/35067088"},{"key":"R33","doi-asserted-by":"crossref","unstructured":"Van Itallie CM\n                      and Anderson JM.\n                      The molecular physiology of tight junction pores.\n                      Physiology\n                      19\n                      : 331\u2013338, 2004.","DOI":"10.1152\/physiol.00027.2004"},{"key":"R34","doi-asserted-by":"crossref","unstructured":"Van Itallie CM\n                      , Fanning AS, and Anderson JM.\n                      Reversal of charge selectivity in cation or anion-selective epithelial lines by expression of different claudins.\n                      Am J Physiol Renal Physiol\n                      285\n                      : F1078\u2013F1084, 2003.","DOI":"10.1152\/ajprenal.00116.2003"},{"key":"R35","doi-asserted-by":"crossref","unstructured":"Van Itallie C\n                      , Rahner C, and Anderson JM.\n                      Regulated expression of claudin 4 decreases paracellular conductance through a selective decrease in sodium permeability.\n                      J Clin Invest\n                      107\n                      : 1319\u20131327, 2001.","DOI":"10.1172\/JCI12464"},{"key":"R36","doi-asserted-by":"crossref","unstructured":"Verrey F\n                      .\n                      Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.\n                      J Membr Biol\n                      138\n                      : 65\u201376, 1994.","DOI":"10.1007\/BF00211070"},{"key":"R37","doi-asserted-by":"crossref","unstructured":"Verrey F\n                      .\n                      Early aldosterone action: toward filling the gap between transcription and transport.\n                      Am J Physiol Renal Physiol\n                      277\n                      : F319\u2013F327, 1999.","DOI":"10.1152\/ajprenal.1999.277.3.F319"},{"key":"R38","doi-asserted-by":"crossref","unstructured":"Wilson FH\n                      , Disse-Nicodeme S, Choate KA, Ishikawa K, Nelson-Williams C, Desitter I, Gunel M, Milford DV, Lipkin GW, Achard JM, Feely MP, Dussol B, Berland Y, Unwin RJ, Mayan H, Simon DB, Farfel Z, Jeunemaitre X, and Lifton RP.\n                      Human hypertension caused by mutations in WNK kinases.\n                      Science\n                      293\n                      : 1107\u20131112, 2001.","DOI":"10.1126\/science.1062844"},{"key":"R39","doi-asserted-by":"crossref","unstructured":"Wilson FH\n                      , Kahle KT, Sabath E, Lalioti MD, Rapson AK, Hoover RS, Hebert SC, Gamba G, and Lifton RP.\n                      Molecular pathogenesis of inherited hypertension with hyperkalemia: the Na-Cl cotransporter is inhibited by wild-type but not mutant WNK4.\n                      Proc Natl Acad Sci USA\n                      100\n                      : 680\u2013684, 2003.","DOI":"10.1073\/pnas.242735399"},{"key":"R40","doi-asserted-by":"crossref","unstructured":"Yamauchi K\n                      , Rai T, Kobayashi K, Sohara E, Suzuki T, Itoh T, Suda S, Hayama A, Sasaki S, and Uchida S.\n                      Disease-causing mutant WNK4 increases paracellular chloride permeability and phosphorylates claudins.\n                      Proc Natl Acad Sci USA\n                      101\n                      : 4690\u20134694, 2004.","DOI":"10.1073\/pnas.0306924101"},{"key":"R41","doi-asserted-by":"crossref","unstructured":"Yu AS\n                      .\n                      Claudins and epithelial paracellular transport: the end of the beginning.\n                      Curr Opin Nephrol Hypertens\n                      12\n                      : 503\u2013509, 2003.","DOI":"10.1097\/00041552-200309000-00004"},{"key":"R42","doi-asserted-by":"crossref","unstructured":"Zettl KS\n                      , Sjaastad MD, Riskin PM, Parry G, Machen TE, and Firestone GL.\n                      Glucocorticoid-induced formation of tight junctions in mouse mammary epithelial cells in vitro.\n                      Proc Natl Acad Sci USA\n                      89\n                      : 9069\u20139073, 1992.","DOI":"10.1073\/pnas.89.19.9069"}],"container-title":["American Journal of Physiology-Cell 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