{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:39:43Z","timestamp":1791995983511,"version":"4.4.0"},"reference-count":56,"publisher":"American Physiological Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:p>\n                    Exercise improves insulin sensitivity and oxidative capacity in skeletal muscles. However, the effect of exercise on substrate oxidation is less clear in obese and type 2 diabetic subjects than in lean subjects. We investigated glucose and lipid metabolism and gene expression after 48 h with low-frequency electrical pulse stimulation (EPS), as an in vitro model of exercise, in cultured myotubes established from lean nondiabetic subjects and severely obese subjects (BMI \u2265 40 kg\/m\n                    <jats:sup>2<\/jats:sup>\n                    ) with and without type 2 diabetes. EPS induced an increase in insulin sensitivity but did not improve lipid oxidation in myotubes from severely obese subjects. Thus, EPS-induced increases in insulin sensitivity and lipid oxidation were positively and negatively correlated to BMI of the subjects, respectively. EPS enhanced oxidative capacity of glucose in myotubes from all subjects. Furthermore, EPS reduced mRNA expression of slow fiber-type marker (MYH7) in myotubes from diabetic subjects; however, the protein expression of this marker was not significantly affected by EPS in either of the donor groups. On the contrary, mRNA levels of interleukin-6 (IL-6) and IL-8 were unaffected by EPS in myotubes from diabetic subjects, while IL-6 mRNA expression was increased in myotubes from nondiabetic subjects. EPS-stimulated mRNA expression levels of MYH7, IL-6, and IL-8 correlated negatively with subjects' HbA\n                    <jats:sub>1c<\/jats:sub>\n                    and\/or fasting plasma glucose, suggesting an effect linked to the diabetic phenotype. Taken together, these data show that myotubes from different donor groups respond differently to EPS, suggesting that this effect may reflect the in vivo characteristics of the donor groups.\n                  <\/jats:p>","DOI":"10.1152\/ajpcell.00314.2014","type":"journal-article","created":{"date-parts":[[2015,1,21]],"date-time":"2015-01-21T22:34:04Z","timestamp":1421879644000},"page":"C548-C556","source":"Crossref","is-referenced-by-count":40,"title":["Myotubes from lean and severely obese subjects with and without type 2 diabetes respond differently to an in vitro model of exercise"],"prefix":"10.1152","volume":"308","author":[{"given":"Yuan Z.","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nata\u0161a","family":"Nikoli\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siril S.","family":"Bakke","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eili T.","family":"Kase","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kari","family":"Guderud","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8ran","family":"Hjelmes\u00e6th","sequence":"additional","affiliation":[{"name":"The Morbid Obesity Center, Vestfold Hospital Trust, T\u00f8nsberg, Norway;"},{"name":"Department of Endocrinology, Morbid Obesity and Preventive Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vigdis","family":"Aas","sequence":"additional","affiliation":[{"name":"Faculty of Health, Oslo and Akershus University College of Applied Sciences, Oslo, Norway; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arild C.","family":"Rustan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Hege","family":"Thoresen","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway;"},{"name":"Department of Pharmacology, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo and Oslo University Hospital, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-013-1655-1"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.077610"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2010.123"},{"key":"B4","author":"Bakke S","journal-title":"PLos One"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-008-1097-6"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00354.2007"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1249\/JES.0b013e31803eae63"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/213986"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-1727"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.2337\/db05-1404"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-013-2592-0"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1139\/y2012-144"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00187.2010"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.12.012"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1124\/pr.109.001560"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3109\/13813455.2013.829105"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M600319-JLR200"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2001.d01-140.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.4.921"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1042\/CS20050207"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2007.01779.x"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2006.036525"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2007.01783.x"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.2337\/diab.44.8.936"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M003319"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00514.2002"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1790"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059972"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-789X.2011.00898.x"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2000.279.5.E1039"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-012-2454-z"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113088"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-2058"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028628"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.06.051"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033203"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2011.06.007"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2011.02.010"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c120033"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e31822f94ac"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.90100.2007"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2012.49"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-010-1764-2"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062008"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110517"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1002\/iub.21"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039657"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00043.2013"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.2337\/db13-1123"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2012-3874"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00237.x"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00776.2009"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00467.2004"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.06.018"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00328.2010"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1007\/s40279-013-0023-3"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00314.2014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T12:35:56Z","timestamp":1718627756000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00314.2014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,1]]},"references-count":56,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,4,1]]}},"alternative-id":["10.1152\/ajpcell.00314.2014"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00314.2014","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725322262.793529439","asserted-by":"object"}]},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,1]]}}}