{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:01:17Z","timestamp":1792738877426,"version":"4.5.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,1,1]]},"abstract":"<jats:p>ATP release induced by hypotonic swelling is an ubiquitous phenomenon in eukaryotic cells, but its underlying mechanisms are poorly defined. A mechanosensitive (MS) ATP channel has been implicated because gadolinium (Gd<jats:sup>3+<\/jats:sup>), an inhibitor of stretch-activated channels, suppressed ATP efflux monitored by luciferase bioluminescence. We examined the effect of Gd<jats:sup>3+<\/jats:sup>on luciferase bioluminescence and on ATP efflux from hypotonically swollen cells. We found that luciferase was inhibited by \u226410 \u03bcM Gd<jats:sup>3+<\/jats:sup>, and this may have contributed to the previously reported inhibition of ATP release. In ATP efflux experiments, luciferase inhibition could be prevented by chelating Gd<jats:sup>3+<\/jats:sup>with EGTA before luminometric ATP determinations. Using this approach, we found that 10\u2013100 \u03bcM Gd<jats:sup>3+<\/jats:sup>, i.e., concentrations typically used to block MS channels, actually stimulated hypotonically induced ATP release from fibroblasts. Inhibition of ATP release required at least 500, 200, or 100 \u03bcM Gd<jats:sup>3+<\/jats:sup>for fibroblasts, A549 cells, and 16HBE14o<jats:sup>\u2212<\/jats:sup>cells, respectively. Such biphasic and cell-specific effects of Gd<jats:sup>3+<\/jats:sup>are most consistent with its action on membrane lipids and membrane-dependent processes such as exocytosis.<\/jats:p>","DOI":"10.1152\/ajpcell.00317.2001","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:06:27Z","timestamp":1368489987000},"page":"C219-C226","source":"Crossref","is-referenced-by-count":81,"title":["Cell swelling-induced ATP release and gadolinium-sensitive channels"],"prefix":"10.1152","volume":"282","author":[{"given":"Francis","family":"Boudreault","sequence":"first","affiliation":[{"name":"Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al and Department of Medicine, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada H2W 1T8"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryszard","family":"Grygorczyk","sequence":"additional","affiliation":[{"name":"Centre hospitalier de l'Universit\u00e9 de Montr\u00e9al and Department of Medicine, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada H2W 1T8"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702933"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(88)83138-2"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000894200"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12324.x"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.150.1.8025763"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005893200"},{"key":"B7","first-page":"862","volume":"295","author":"Burnstock G","year":"2000","journal-title":"J Pharmacol Exp Ther"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.2.C619"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(99)00125-X"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.10.1.7507342"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/BF00238414"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.3.C577"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)76155-3"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46217-X"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.3.C1058"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007010200"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.2.685"},{"key":"B19","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0031-6997(25)06942-X","volume":"48","author":"Hamill OP","year":"1996","journal-title":"Pharmacol Rev"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.114.4.525"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0759e.x"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24348"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101500200"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.7174"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.19.10465"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.277.6.G1222"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.2.C295"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.5.C1391"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.12020"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1126\/science.2466333"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3163"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00101.x"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00317.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T09:53:51Z","timestamp":1746006831000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00317.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,1]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1,1]]}},"alternative-id":["10.1152\/ajpcell.00317.2001"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00317.2001","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,1]]}}}