{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T06:01:18Z","timestamp":1759384878901,"version":"3.40.5"},"reference-count":55,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2020,4,1]]},"abstract":"<jats:p> Carotid body (CB) type I cells sense the blood Po<jats:sub>2<\/jats:sub> and generate a nervous signal for stimulating ventilation and circulation when blood oxygen levels decline. Three oxygen-sensing enzyme complexes may be used for this purpose: 1) mitochondrial electron transport chain metabolism, 2) heme oxygenase 2 (HO-2)-generating CO, and\/or 3) an NAD(P)H oxidase (NOX). We hypothesize that intracellular redox changes are the link between the sensor and nervous signals. To test this hypothesis type I cell autofluorescence of flavoproteins (Fp) and NAD(P)H within the mouse CB ex vivo was recorded as Fp\/(Fp+NAD(P)H) redox ratio. CB type I cell redox ratio transiently declined with the onset of hypoxia. Upon reoxygenation, CB type I cells showed a significantly increased redox ratio. As a control organ, the non-oxygen-sensing sympathetic superior cervical ganglion (SCG) showed a continuously reduced redox ratio upon hypoxia. CN<jats:sup>\u2212<\/jats:sup>, diphenyleneiodonium, or reactive oxygen species influenced chemoreceptor discharge (CND) with subsequent loss of O<jats:sub>2<\/jats:sub> sensitivity and inhibited hypoxic Fp reduction only in the CB but not in SCG Fp, indicating a specific role of Fp in the oxygen-sensing process. Hypoxia-induced changes in CB type I cell redox ratio affected peptidyl prolyl isomerase Pin1, which is believed to colocalize with the NADPH oxidase subunit p47<jats:sub>phox<\/jats:sub> in the cell membrane to trigger the opening of potassium channels. We postulate that hypoxia-induced changes in the Fp-mediated redox ratio of the CB regulate the Pin1\/p47<jats:sub>phox<\/jats:sub> tandem to alter type I cell potassium channels and therewith CND. <\/jats:p>","DOI":"10.1152\/ajpcell.00320.2019","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T21:14:30Z","timestamp":1579727670000},"page":"C719-C731","source":"Crossref","is-referenced-by-count":11,"title":["Carotid body type I cells engage flavoprotein and Pin1 for oxygen sensing"],"prefix":"10.1152","volume":"318","author":[{"given":"Andr\u00e9","family":"Bernardini","sequence":"first","affiliation":[{"name":"Institute of Physiology, University of Duisburg-Essen, Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Wolf","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Duisburg-Essen, Essen, 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