{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:30:18Z","timestamp":1785364218337,"version":"3.55.0"},"reference-count":66,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2013,9,15]]},"abstract":"<jats:p> The mechanisms governing maintenance of quiescence during pregnancy remain largely unknown. The current study characterizes a stretch-activated, tetraethylammonium-insensitive K<jats:sup>+<\/jats:sup> current in smooth muscle cells isolated from pregnant human myometrium. This study hypothesizes that these K<jats:sup>+<\/jats:sup> currents can be attributed to TREK-1 and that upregulation of this channel during pregnancy assists with the maintenance of a negative cell membrane potential, conceivably contributing to uterine quiescence until full term. The results of this study demonstrate that, in pregnant human myometrial cells, outward currents at 80 mV increased from 4.8 \u00b1 1.5 to 19.4 \u00b1 7.5 pA\/pF and from 3.0 \u00b1 0.8 to 11.8 \u00b1 2.7 pA\/pF with application of arachidonic acid (AA) and NaHCO<jats:sub>3<\/jats:sub>, respectively, causing intracellular acidification. Similarly, outward currents were inhibited following application of 10 \u03bcM fluphenazine by 51.2 \u00b1 9.8% after activation by AA and by 73.9 \u00b1 4.2% after activation by NaHCO<jats:sub>3<\/jats:sub>. In human embryonic kidney (HEK-293) cells stably expressing TREK-1, outward currents at 80 mV increased from 91.0 \u00b1 23.8 to 247.5 \u00b1 73.3 pA\/pF and from 34.8 \u00b1 8.9 to 218.6 \u00b1 45.0 pA\/pF with application of AA and NaHCO<jats:sub>3<\/jats:sub>, respectively. Correspondingly, outward currents were inhibited 89.5 \u00b1 2.3% by 10 \u03bcM fluphenazine following activation by AA and by 91.6 \u00b1 3.4% following activation by NaHCO<jats:sub>3<\/jats:sub>. Moreover, currents in human myometrial cells were activated by stretch and were reduced by transfection with small interfering RNA or extracellular acidification. Understanding gestational regulation of expression and gating of TREK-1 channels could be important in determining appropriate maintenance of uterine quiescence during pregnancy. <\/jats:p>","DOI":"10.1152\/ajpcell.00324.2012","type":"journal-article","created":{"date-parts":[[2013,6,27]],"date-time":"2013-06-27T04:37:03Z","timestamp":1372307823000},"page":"C632-C642","source":"Crossref","is-referenced-by-count":23,"title":["TREK-1 currents in smooth muscle cells from pregnant human myometrium"],"prefix":"10.1152","volume":"305","author":[{"given":"Nathanael S.","family":"Heyman","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Center for Molecular Medicine, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad L.","family":"Cowles","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular Medicine, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott D.","family":"Barnett","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular Medicine, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ying","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular Medicine, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Cullison","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular Medicine, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cherie A.","family":"Singer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular Medicine, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Normand","family":"Leblanc","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Center for Molecular Medicine, University of Nevada School of Medicine, Reno, Nevada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain L. 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