{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:26:53Z","timestamp":1791923213290,"version":"4.3.4"},"reference-count":54,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2012,3,15]]},"abstract":"<jats:p> Acid-sensing ion channel 1 (ASIC1) is a newly characterized contributor to store-operated Ca<jats:sup>2+<\/jats:sup> entry (SOCE) in pulmonary vascular smooth muscle (VSM). Since SOCE is implicated in elevated basal VSM intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and augmented vasoconstriction in chronic hypoxia (CH)-induced pulmonary hypertension, we hypothesized that ASIC1 contributes to these responses. To test this hypothesis, we examined effects of the specific pharmacologic ASIC1a inhibitor, psalmotoxin 1 (PcTX1), on vasoconstrictor and vessel wall [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> responses to UTP and KCl (depolarizing stimulus) in fura-2-loaded, pressurized small pulmonary arteries from control and CH (4 wk at 0.5 atm) Wistar rats. PcTX1 had no effect on basal vessel wall [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, but attenuated vasoconstriction and increases in vessel wall [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> to UTP in arteries from control and CH rats; normalizing responses between groups. In contrast, responses to the depolarizing stimulus, KCl, were unaffected by CH exposure or PcTX1. Upon examining potential Ca<jats:sup>2+<\/jats:sup> influx mechanisms, we found that PcTX1 prevented augmented SOCE following CH. Exposure to CH resulted in a significant increase in pulmonary arterial ASIC1 protein. This study supports a novel role of ASIC1 in elevated receptor-stimulated vasoconstriction following CH which is likely mediated through increased ASIC1 expression and SOCE. <\/jats:p>","DOI":"10.1152\/ajpcell.00332.2011","type":"journal-article","created":{"date-parts":[[2011,12,29]],"date-time":"2011-12-29T02:56:54Z","timestamp":1325127414000},"page":"C931-C940","source":"Crossref","is-referenced-by-count":45,"title":["Chronic hypoxia upregulates pulmonary arterial ASIC1: a novel mechanism of enhanced store-operated Ca<sup>2+<\/sup> entry and receptor-dependent vasoconstriction"],"prefix":"10.1152","volume":"302","author":[{"given":"Nikki L.","family":"Jernigan","sequence":"first","affiliation":[{"name":"Vascular Physiology Group, Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay M.","family":"Herbert","sequence":"additional","affiliation":[{"name":"Vascular Physiology Group, Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjimen R.","family":"Walker","sequence":"additional","affiliation":[{"name":"Vascular Physiology Group, Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Resta","sequence":"additional","affiliation":[{"name":"Vascular Physiology Group, Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/830320"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00101.2006"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.281.1.L193"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00253.2007"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000144465.56360.ad"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/nature04702"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01380.2007"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.2.H746"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00333.2006"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2007.08.003"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2009.01.007"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00308.2004"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00177.2006"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00020.2009"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00328.2002"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00355.2007"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00007.2002"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1046\/j.1540-8167.2002.00493.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000138952.16382.ad"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R700011200"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.05.055"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00247.2011"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00063.2009"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00328.2009"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704468"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00050.2003"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00819.2004"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.172254"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00548.2009"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.280.4.L801"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.157.4.9704106"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00327.2006"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-1-00669.x"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200502019"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.279.5.L884"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1999.277.3.L431"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1080\/10739680600930305"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1994.266.1.H365"},{"key":"B39","first-page":"1","volume":"22","author":"Snow JB","year":"2009","journal-title":"Microcirculation"},{"key":"B40","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.1111\/j.1582-4934.2010.01097.x","volume":"14","author":"Song M","year":"2010","journal-title":"J Cell Mol Med"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1982.242.5.H907"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127883"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-3401-0_19"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/386173a0"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-10-123"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000227551.68124.98"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1080\/10739680802653150"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00044.2005"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00449.2004"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.08.026"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308636100"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.98.14.1400"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00214.2006"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1038\/nature04147"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00332.2011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T16:55:12Z","timestamp":1718643312000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00332.2011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,15]]},"references-count":54,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,3,15]]}},"alternative-id":["10.1152\/ajpcell.00332.2011"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00332.2011","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,15]]}}}