{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:29:35Z","timestamp":1790810975214,"version":"4.1.0"},"reference-count":62,"publisher":"American Physiological Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2012,10,15]]},"abstract":"<jats:p> The polarized organization of epithelial cells is required for vectorial solute transport and may be altered in renal cystic diseases. Vesicle integral protein of 17 kDa (VIP17\/MAL) is involved in apical vesicle transport. VIP17\/MAL overexpression in vivo results in renal cystogenesis of unknown etiology. Renal cystogenesis can occur as a consequence of defects of the primary cilium. To explore the role of VIP17\/MAL in renal cystogenesis and ciliogenesis, we examined the polarization and ciliary morphology of wild-type and VIP17\/MAL overexpressing Madin-Darby canine kidney renal epithelial cells grown in two-dimensional (2D) and three-dimensional (3D) cyst culture. VIP17\/MAL is apically localized when expressed in cells maintained in 2D and 3D culture. VIP17\/MAL overexpressing cells produce more multilumen cysts compared with controls. While the distributions of basolateral markers are not affected, VIP17\/MAL expression results in aberrant sorting of the apical marker gp135 to the primary cilium. VIP17\/MAL overexpression is also associated with shortened or absent cilia. Immunofluorescence analysis performed on kidney sections from VIP17\/MAL transgenic mice also demonstrates fewer and shortened cilia within dilated lumens ( P &lt; 0.01). These studies demonstrate that VIP17\/MAL overexpression results in abnormal cilium and cyst development, in vitro and in vivo, suggesting that VIP17\/MAL overexpressing mice may develop cysts secondary to a ciliary defect. <\/jats:p>","DOI":"10.1152\/ajpcell.00338.2011","type":"journal-article","created":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T01:53:22Z","timestamp":1345082002000},"page":"C862-C871","source":"Crossref","is-referenced-by-count":10,"title":["VIP17\/MAL expression modulates epithelial cyst formation and ciliogenesis"],"prefix":"10.1152","volume":"303","author":[{"given":"Vinita","family":"Takiar","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavita","family":"Mistry","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Carmosino","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Schaeren-Wiemers","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, University Hospital Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Caplan","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.7.1997"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.4.1141"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb02194.x"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E07-02-0142"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2008.06.023"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E10-05-0456"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.03.004"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201006173"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.11.6241"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200412021"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200609096"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.08.025"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V1061337"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001361"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200910096"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-02-0133"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201009001"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0751927.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-13-04901.1998"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-7-8"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1126\/science.1191054"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5111"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708023104"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.09.185"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490420316"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/mc.10034"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn208"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1042\/BC20100150"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0836980100"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E09-02-0142"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.15.6007"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.4.5875"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200407072"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200907126"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199811)28:11<3675::AID-IMMU3675>3.0.CO;2-5"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6530"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0901-831"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12662-1"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(02)02410-8"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.08.007"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.18.8414"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.1.141"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M410697200"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609324103"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E03-05-0296"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204825"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90357-K"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1038\/ng1552"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124534"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1999.276.2.F210"},{"key":"B51","author":"Takiar V","year":"2010","journal-title":"Biochim Biophys Acta"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp272"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.015495"},{"key":"B54","first-page":"152","volume":"18","author":"van Adelsberg J","year":"1998","journal-title":"Semin Nephrol"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608291103"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000113793.12558.1D"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1038\/ng0803-355"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.1.73"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00273.2001"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1002\/path.2392"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)01396-2"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.238469"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00338.2011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T16:54:01Z","timestamp":1718643241000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00338.2011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,15]]},"references-count":62,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2012,10,15]]}},"alternative-id":["10.1152\/ajpcell.00338.2011"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00338.2011","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,15]]}}}