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Still, the lack of a viable in vitro culture of Brunner\u2019s glands has hampered additional studies of the functional consequences of GLP-1R activation. In this study, we established a procedure to isolate and culture cells derived from murine Brunner\u2019s glands. The isolated glandular cells retained functional GLP-1R expression in culture, making this in vitro system suitable for the study of GLP-1R activation. We found that cells derived from the Brunner\u2019s glands of mice pretreated with semaglutide contained significantly more mucus compared with Brunner\u2019s glands from vehicle-treated mice. Our data suggest a protective intestinal response upon semaglutide treatment, but further studies are required to leverage the full potential of cultured Brunner\u2019s gland cells.<\/jats:p>","DOI":"10.1152\/ajpcell.00345.2021","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T15:45:57Z","timestamp":1650469557000},"page":"C1260-C1269","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["In vitro cell cultures of Brunner\u2019s glands from male mouse to study GLP-1 receptor function"],"prefix":"10.1152","volume":"322","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0532-3154","authenticated-orcid":true,"given":"Louise M.","family":"Voetmann","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark"},{"name":"Global Drug Discovery, Novo Nordisk A\/S, 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