{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:28:36Z","timestamp":1790292516873,"version":"4.1.0"},"reference-count":59,"publisher":"American Physiological Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2014,4,15]]},"abstract":"<jats:p>Melatonin secretion from the pineal gland is triggered by norepinephrine released from sympathetic terminals at night. In contrast, cholinergic and parasympathetic inputs, by activating nicotinic cholinergic receptors (nAChR), have been suggested to counterbalance the noradrenergic input. Here we investigated whether adrenergic signaling regulates nAChR channels in rat pinealocytes. Acetylcholine or the selective nicotinic receptor agonist 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP) activated large nAChR currents in whole cell patch-clamp experiments. Norepinephrine (NE) reduced the nAChR currents, an effect partially mimicked by a \u03b2-adrenergic receptor agonist, isoproterenol, and blocked by a \u03b2-adrenergic receptor antagonist, propranolol. Increasing intracellular cAMP levels using membrane-permeable 8-bromoadenosine (8-Br)-cAMP or 5,6-dichlorobenzimidazole riboside-3\u2032,5\u2032-cyclic monophosphorothioate (cBIMPS) also reduced nAChR activity, mimicking the effects of NE and isoproterenol. Further, removal of ATP from the intracellular pipette solution blocked the reduction of nAChR currents, suggesting involvement of protein kinases. Indeed protein kinase A inhibitors, H-89 and Rp-cAMPS, blocked the modulation of nAChR by adrenergic stimulation. After the downmodulation by NE, nAChR channels mediated a smaller Ca<jats:sup>2+<\/jats:sup>influx and less membrane depolarization from the resting potential. Together these results suggest that NE released from sympathetic terminals at night attenuates nicotinic cholinergic signaling.<\/jats:p>","DOI":"10.1152\/ajpcell.00354.2013","type":"journal-article","created":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T06:32:45Z","timestamp":1392877965000},"page":"C726-C735","source":"Crossref","is-referenced-by-count":9,"title":["Modulation of nicotinic receptor channels by adrenergic stimulation in rat pinealocytes"],"prefix":"10.1152","volume":"306","author":[{"given":"Jin-Young","family":"Yoon","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Washington, Seattle, Washington; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Ryoung","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Washington, Seattle, Washington; 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