{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T09:31:40Z","timestamp":1767951100214,"version":"3.49.0"},"reference-count":21,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:p> In smooth muscle, large-conductance Ca<jats:sup>2+<\/jats:sup>- and voltage-activated K<jats:sup>+<\/jats:sup> channels from the gene KCNMA (maxi-K channels) generate isoforms with disparate responses to contractile stimuli. We previously showed that the human myometrium expresses high levels of the splice variant of the maxi-K channel containing a 44-amino acid insertion (mK44). The studies presented here demonstrate that nardilysin convertase, a Zn<jats:sup>2+<\/jats:sup>-dependent metalloprotease of the insulinase family, regulates the plasma membrane expression of mK44 and its response to increases in intracellular Ca<jats:sup>2+<\/jats:sup>. We show that nardilysin convertase isoform 1 is present in human myometrium and colocalizes with mK44. Studies indicate that nardilysin convertase regulates 1) retention of the mK44 COOH-terminal fragment in the endoplasmic reticulum in quiescent myometrial smooth muscle and 2) Ca<jats:sup>2+<\/jats:sup>-induced translocation of mK44 to the plasma membrane. In mouse fibroblasts, nardilysin convertase significantly attenuates mK44-dependent current. In human myometrial smooth muscle cells, inhibition of nardilysin convertase promotes membrane localization of mK44 and an increase in maxi-K current. Overall, our data indicate that, in human myometrium, nardilysin convertase and mK44 channels are a part of the molecular mechanism that regulates the excitability of smooth muscle cells. <\/jats:p>","DOI":"10.1152\/ajpcell.00357.2008","type":"journal-article","created":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T01:34:30Z","timestamp":1230773670000},"page":"C433-C440","source":"Crossref","is-referenced-by-count":9,"title":["Nardilysin convertase regulates the function of the maxi-K channel isoform mK44 in human myometrium"],"prefix":"10.1152","volume":"296","author":[{"given":"Victoria P.","family":"Korovkina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan J.","family":"Stamnes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam M.","family":"Brainard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah K.","family":"England","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Adebamiro A, Cheng Y, Rao US, Danahay H, Bridges RJ. 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