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Application of ATP also evoked a reversible inward current at V<jats:sub>h<\/jats:sub>= \u221250 mV, but the current showed two components. A fast component with a short latency was largely reduced when N-methyl-d-glucamine (NMDG) replaced extracellular sodium, implying a P2X-like ionotropic conductance. The second component had a longer latency (average 1.1 s) and was presumably activated by metabotropic P2Y-like receptors. The second component of ATP-evoked current shared similar characteristics with the responses evoked by ACh: the current reversed near 0 mV, displayed inward rectification, could be carried by NMDG, and was insensitive to extracellular and intracellular calcium. This ACh-\/ATP-evoked conductance was reversibly inhibited by preapplication of ionomycin. These results suggest that muscarinic receptors and purinergic metabotropic receptors activate a similar large nonselective cation conductance via a common intracellular pathway in SGNs, a candidate mechanism to regulate neuronal excitability of SGNs.<\/jats:p>","DOI":"10.1152\/ajpcell.00364.2001","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:08:22Z","timestamp":1368490102000},"page":"C1121-C1135","source":"Crossref","is-referenced-by-count":32,"title":["Nonselective cation conductance activated by muscarinic and purinergic receptors in rat spiral ganglion neurons"],"prefix":"10.1152","volume":"282","author":[{"given":"Ken","family":"Ito","sequence":"first","affiliation":[{"name":"Laboratoire de Biologie Cellulaire et Mol\u00e9culaire de l'Audition, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale EMI 99-27, Universit\u00e9 de Bordeaux 2, H\u00f4pital Pellegrin, 33076 Bordeaux, France; and"},{"name":"Department of 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